SSBD:database

Project

Dataset ID

Fig2_onephoton_mito_3D_SPASIM

Kind

Image data

Title

3D rendered images of mitochondria in living HeLa cells with SPASIM for single-photon activation

Description

The 3D rendered images of mitochondria visualized by Skylan-NS in living HeLa cells were captured with selective-plane-activation structured illumination microscopy (SPA-SIM) using single-photon activation. The images with SPA-SIM showed higher contrast and better visualization of the mitochondria distributed throughout the cell. Skylan-NS is a photoswitchable green fluorescent protein. CW laser is used for single-photon activation, which is more efficient and allows measurement with higher temporal resolution.

Folder Name

Fig2_onephoton_mito_3D_SPASIM

File Format

tif

File Size

1.3GB

File Separation Method

-

Figure Number

Figure2i

Dimensions

2048x2048x81x1x1

X Scale

141.9 micrometer

Y Scale

141.9 micrometer

Z Scale

250 nanometer

T Scale

-

Channel

1 channel

Quantitative Data

-

Description

Mitochondria were visualized using mitochondria-targeted Skylan-NS in living HeLa cell spheroids.

Organism

Strain

-

Cell

-

Cell line

HeLa-S3 cell (CLO:0003699)

MeSH

Intrinsic Variable

-

Extrinsic Variable

HeLa cells were electroporated with Skylan-NS plasmids

Anatomical Entity

-

UBERON

-

Biological Process

-

Cellular Component

Molecular Function

-

MeSH Anatomy

MeSH BP

MeSH CC

MeSH MF

Imaging Method

Detection Method

detection of photons

Visualization Method

Skylan-NS

Illumination Method

light-sheet illumination

Source of Contrast

-

Imaged Parameter

-

Sample Preparation Method

living cell

MeSH

Body

-

Module

-

Light Source

405 nm light sheet illumination

Detector

Hamamatsu Photonics ORCA-Flash 4.0 v3

Objective

Nikon CFI75 Apo 25XC W, Special Optics 54-10-7

Filter Set

-

Dichroic

Di03-R488-t3-25x36, RazorEdge LongPass488