SSBD:database

448-Temma-HeLaSPASIM/Fig2_onephoton_mito_3D_3DSIM thumbnail

448-Temma-HeLaSPASIM/Fig2_onephoton_mito_3D_3DSIM

Author reviewed
3D rendered images of mitochondria in living HeLa cells with 3DSIM for single-photon activation
SSBD:OMERO.gallery (Project) SSBD:OMERO (Project) SSBD:OMERO.gallery (Dataset) SSBD:OMERO (Dataset)
SSBD:downloads
Project
448-Temma-HeLaSPASIM
Dataset ID
Fig2_onephoton_mito_3D_3DSIM
Kind
Image data
Title
3D rendered images of mitochondria in living HeLa cells with 3DSIM for single-photon activation
Description
The 3D rendered images of mitochondria visualized by Skylan-NS in living HeLa cells were captured with three-dimensional structured illumination microscopy (3DSIM) using single-photon activation. The distribution of mitochondria by the background in 3DSIM was obscured. Skylan-NS is a photoswitchable green fluorescent protein. CW laser is used for single-photon activation, which is more efficient and allows measurement with higher temporal resolution.
Folder Name
Fig2_onephoton_mito_3D_3DSIM
File Format
tif
File Size
1.3GB
File Separation Method
-
Figure Number
Figure2j
Dimensions
2048x2048x80x1x1
X Scale
141.9 micrometer
Y Scale
141.9 micrometer
Z Scale
250 nanometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Mitochondria were visualized using mitochondria-targeted Skylan-NS in living HeLa cell spheroids.
Organism
Homo sapiens
(NCBITaxon:9606)
Strain
-
Cell
-
Cell line
HeLa-S3 cell (CLO:0003699)
MeSH
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Mitochondria (D008928)
Intrinsic Variable
-
Extrinsic Variable
HeLa cells were electroporated with Skylan-NS plasmids

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
mitochondrion (GO:0005739)
Molecular Function
-
MeSH Anatomy
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Mitochondria (D008928)
MeSH BP
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Mitochondria (D008928)
MeSH CC
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Mitochondria (D008928)
MeSH MF
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Mitochondria (D008928)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
structured illumination microscopy
(FBbi:00000332)
Detection Method
detection of photons
Visualization Method
Skylan-NS
Illumination Method
wide-field illumination
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
living cell
MeSH
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Mitochondria (D008928)

Instrument

Body
-
Module
-
Light Source
405 nm wide-field illumination
Detector
Hamamatsu Photonics ORCA-Flash 4.0 v3
Objective
Nikon CFI75 Apo 25XC W, Special Optics 54-10-7
Filter Set
-
Dichroic
Di03-R488-t3-25x36, RazorEdge LongPass488