439-Takayama-dynamicsEGFR/Fig1A_MbCDandChol_withoutEGF
Author reviewedTime-lapse TIRF images of EGFR-GFP on MbCD and cholesterol treated-CHO-K1 cells without EGF stimulation
Project
Dataset ID
Fig1A_MbCDandChol_withoutEGF
Kind
Image data
Title
Time-lapse TIRF images of EGFR-GFP on MbCD and cholesterol treated-CHO-K1 cells without EGF stimulation
Description
Time-lapse TIRF (total internal reflection fluorescence) images of EGFR-GFP on CHO-K1 cells without EGF stimulation. Cholesterol was added to recover it after its depletion from the plasma membrane by MbCD (methyl-beta-cyclodextrin).
Folder Name
Fig1A_MbCDandChol_withoutEGF
File Format
.tif
File Size
34MB
File Separation Method
-
Figure Number
Fig1A
Dimensions
768x768x1x1x30
X Scale
72 nanometer
Y Scale
72 nanometer
Z Scale
-
T Scale
0.03 seconds,
30 frames per second
Channel
1 channel
Quantitative Data
Yes
Paper(s)
Biosample
Description
Methyl-beta-cyclodextrin and water-soluble cholesterol administered CHO-K1 cells without EGF treatment.
Organism
Cricetulus griseus (NCBITaxon:10029)
Strain
-
Cell
-
Cell line
CHO-K1 cell (CLO:0002462)
Intrinsic Variable
-
Extrinsic Variable
Water-soluble cholesterol was applied to cells treated with methyl-beta-cyclodextrin in order to reload the cholesterol to cell membranes. Cells were treated with 30 nM EGF during imaging.
Ontology
Anatomical Entity
-
UBERON
cell (CL:0000000)
Biological Process
regulation of epidermal growth factor receptor signaling pathway (GO:0042058)
receptor clustering (GO:0043113)
Cellular Component
plasma membrane (GO:0005886)
receptor complex (GO:0043235)
Molecular Function
epidermal growth factor receptor activity (GO:0005006)
intracellular signal transduction (GO:0035556)
MeSH Anatomy
Genetics
Gene
-
Protein / Probe
Epidermal growth factor receptor
Method / Genotype
transfection
Treatments
Reagent / Compound
methyl-beta-cyclodextrin
Concentration
5 millimolar
Fold Dilution
-
Reagent / Compound
cholesterol
Concentration
500 microgram per milliliter
Fold Dilution
-
Imaging Method
Imaging Method
evanescent wave microscopy (FBbi:00000617)
Detection Method
-
Visualization Method
Green fluorescent protein from Aequorea
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
Instrument
Body
Nikon Ti2-E
Module
-
Light Source
-
Detector
Hamamatsu C13440-20CU
Objective
-
Filter Set
-
Dichroic
-