436-Yano-MNincorporate/Video5e_MicronucleiVivo
Author reviewedTwo-photon in vivo images of the Cx3cr1-EGFP;H2B-mCherry mouse brain
Project
Dataset ID
Video5e_MicronucleiVivo
Kind
Image data
Title
Two-photon in vivo images of the Cx3cr1-EGFP;H2B-mCherry mouse brain
Description
Z-series and time-lapse images of mouse brain expressing EGFP in microglia, and H2B-mCherry in nucleus. ch1: Cx3cr1-EGFP, ch2: H2B-mCherry
Folder Name
Video5e_MicronucleiVivo
File Format
.nd2
File Size
2.3GB
File Separation Method
-
Figure Number
-
Dimensions
512x512x39x2x61
X Scale
0.497 micrometer
Y Scale
0.497 micrometer
Z Scale
1 micrometer
T Scale
1 minute
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
Live and anesthetized mouse brain of lines with
Cx3cr1-EGFP+/−;H2B-mCherry+/− mice at P9-P14
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron microglial cell (CL:0000129)
Cell line
-
MeSH
Intrinsic Variable
The transgenic mouse lines, Cx3
cr1-EGFP+/−;H2B-mCherry+/−
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
brain (UBERON:0000955)
Biological Process
cell morphogenesis (GO:0000902)
Cellular Component
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
gene knock in / B6.129P2(Cg)-Cx3cr1^{tm1Litt}/J
R26-H2B-mCherry
Gene
-
Protein / Probe
Histone H2B type 1-J
Method / Genotype
transgenic / B6.129P2(Cg)-Cx3cr1^{tm1Litt}/J
R26-H2B-mCherry
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
EGFP
mCherry
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Nikon NIS-Elements
Module
-
Light Source
-
Detector
-
Objective
Nikon Apo LWDx25/1.10w
Filter Set
-
Dichroic
-