436-Yano-MNincorporate/Video1_MicronucleiSlice
Author reviewedTime-lapse images of Cx3cr1-EGFP;H2B-mCherry cortical slice culture from P3 mouse
Project
Dataset ID
Video1_MicronucleiSlice
Kind
Image data
Title
Time-lapse images of Cx3cr1-EGFP;H2B-mCherry cortical slice culture from P3 mouse
Description
Time-lapse images of P3 (postnatal day3) mouse brain slice expressing EGFP in microglia, and H2B-mCherry in nucleus.
Folder Name
Video1_MicronucleiSlice
File Format
.jpg
File Size
1.1MB
File Separation Method
channels and each time point are separated into files
Figure Number
Extended Data Fig3
Dimensions
512x512x1x2x24
X Scale
0.4 micrometer
Y Scale
0.4 micrometer
Z Scale
-
T Scale
5 minutes
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
Cortical slice culture of forebrains isolated from Cx3cr1-EGFP+/−;H2B-mCherry+/− mice at P3
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron microglial cell (CL:0000129)
Cell line
-
MeSH
Intrinsic Variable
The transgenic mouse lines, Cx3
cr1-EGFP+/−;H2B-mCherry+/−
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
brain (UBERON:0000955)
Biological Process
cell morphogenesis (GO:0000902)
Cellular Component
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
gene knock in / B6.129P2(Cg)-Cx3cr1^{tm1Litt}/J
R26-H2B-mCherry
Gene
-
Protein / Probe
Histone H2B type 1-J
Method / Genotype
transgenic / B6.129P2(Cg)-Cx3cr1^{tm1Litt}/J
R26-H2B-mCherry
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
EGFP
mCherry
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Yokogawa CSU-x1
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident LUMPLanFL Nx40/0.80 W
Filter Set
-
Dichroic
-