SSBD:database

422-Asai-KinetochoreBeads/FigS3C_mitosis_17

Author reviewed

Z-series and time-lapse images of chromosomes and NDC80-NUF2 beads in embryo during the first mitosis

SSBD:downloads

Project

422-Asai-KinetochoreBeads

Dataset ID

FigS3C_mitosis_17

Kind

Image data

Title

Z-series and time-lapse images of chromosomes and NDC80-NUF2 beads in embryo during the first mitosis

Description

Z-series and time-lapse images of chromosomes and NDC80-NUF2 beads in embryo during the first mitosis. NDC80-NUF2 (NDC80-NUF2 heterodimer) beads were formed in the embryo as follows; The authors microinjected mRNAs encoding C-terminally green fluorescent protein–tagged NDC80 (NDC80-GFP) and NUF2, followed by anti-GFP–conjugated microbeads into the cytoplasm. The mRNA for H2B-mCherry was also injected to visualize the chromosomes.

Folder Name

FigS3C_mitosis_17

File Format

lsm

File Size

8.6GB

File Separation Method

Figure Number

FigS3C

Dimensions

512x512x25x3x210

X Scale

0.07 micrometer

Y Scale

0.07 micrometer

Z Scale

1.25 micrometer

T Scale

3 minutes

Channel

3 channel

Quantitative Data

Paper(s)

Biosample

Description

Mouse embryo which was microinjected mRNAs encoding C-terminally green fluorescent protein–tagged NDC80 (NDC80-GFP) and NUF2, followed by anti-GFP–conjugated microbeads (1.7 to 2.6 μm in diameter), into the cytoplasm

Organism

Mus musculus (NCBITaxon:10090)

Strain

B6D2F1 (https://www.jax.or.jp/product-and-service/rm/detail/bdf1)

Cell

embryonic cell (metazoa) (CL:0002321)

Cell line

MeSH

Embryo, Mammalian (D004622)

Microscopy, Confocal (D018613)

Mitosis (D008938)

Kinetochores (D018386)

Chromosome Segregation (D020090)

Chromosomes (D002875)

Intrinsic Variable

Extrinsic Variable

Mouse embryo was microinjected mRNAs encoding C-terminally green fluorescent protein–tagged NDC80 (NDC80-GFP) and NUF2, followed by anti-GFP–conjugated microbeads (1.7 to 2.6 μm in diameter). The H2B-mCherry mRNA was also injexcted.

Ontology

Anatomical Entity

UBERON

embryo (UBERON:0000922)

Biological Process

nuclear chromosome segregation (GO:0098813)

kinetochore assembly (GO:0051382)

Cellular Component

kinetochore (GO:0000776)

chromosome (GO:0005694)

Molecular Function

MeSH Anatomy

Embryo, Mammalian (D004622)

Microscopy, Confocal (D018613)

Mitosis (D008938)

Kinetochores (D018386)

Chromosome Segregation (D020090)

Chromosomes (D002875)

MeSH BP

Embryo, Mammalian (D004622)

Microscopy, Confocal (D018613)

Mitosis (D008938)

Kinetochores (D018386)

Chromosome Segregation (D020090)

Chromosomes (D002875)

MeSH CC

Embryo, Mammalian (D004622)

Microscopy, Confocal (D018613)

Mitosis (D008938)

Kinetochores (D018386)

Chromosome Segregation (D020090)

Chromosomes (D002875)

MeSH MF

Embryo, Mammalian (D004622)

Microscopy, Confocal (D018613)

Mitosis (D008938)

Kinetochores (D018386)

Chromosome Segregation (D020090)

Chromosomes (D002875)

Genetics

Gene

Protein / Probe

Kinetochore protein NDC80 homolog, Kinetochore protein Nuf2

Method / Genotype

injection

Gene

Protein / Probe

Histone H2B type 1-J

Method / Genotype

injection

Treatments

Reagent / Compound

Concentration

Fold Dilution

Imaging Method

confocal microscopy (FBbi:00000251)

time lapse microscopy (FBbi:00000249)

Detection Method

Visualization Method

Green fluorescent protein from Aequorea, mCherry

Illumination Method

Source of Contrast

distribution of a specific protein

Imaged Parameter

fluorescence emission

Sample Preparation Method

living tissue

MeSH

Embryo, Mammalian (D004622)

Microscopy, Confocal (D018613)

Mitosis (D008938)

Kinetochores (D018386)

Chromosome Segregation (D020090)

Chromosomes (D002875)

Instrument

Body

Zeiss LSM880

Module

Light Source

Detector

Objective

Zeiss 40×C-Apochromat 1.2NA

Filter Set

Dichroic