422-Asai-KinetochoreBeads/FigS3C_meiosis_8
Author reviewed
Z-series images of chromosomes and NDC80-NUF2 beads in oocyte during meiosis II
Dataset ID
FigS3C_meiosis_8
Title
Z-series images of chromosomes and NDC80-NUF2 beads in oocyte during meiosis II
Description
Z-series images of chromosomes and NDC80-NUF2 beads in mouse oocyte during meiosis II. NDC80-NUF2 (NDC80-NUF2 heterodimer) beads were formed in the oocyte as follows; The authors microinjected mRNAs encoding
C-terminally green fluorescent protein–tagged
NDC80 (NDC80-GFP) and NUF2, followed by
anti-GFP–conjugated microbeads into the cytoplasm. The mRNA for H2B-mCherry was also injected to visualize the chromosomes.
channel1; GFP, channel2; mCherry, channel3;bright field.
Folder Name
FigS3C_meiosis_8
File Separation Method
2 series
Dimensions
256x256x30x3x1,
128x128x15x3x1
X Scale
0.24 micrometer,
0.47 micrometer
Y Scale
0.24 micrometer,
0.47 micrometer
Biosample
Description
Mouse oocyte which was microinjected mRNAs encoding
C-terminally green fluorescent protein–tagged
NDC80 (NDC80-GFP) and NUF2, followed by
anti-GFP–conjugated microbeads (1.7 to 2.6 μm in diameter), into the cytoplasm
Extrinsic Variable
Mouse oocyte was microinjected mRNAs encoding
C-terminally green fluorescent protein–tagged
NDC80 (NDC80-GFP) and NUF2, followed by
anti-GFP–conjugated microbeads (1.7 to 2.6 μm in diameter). The H2B-mCherry mRNA was also injexcted.
Ontology
Biological Process
female meiosis chromosome segregation (
GO:0016321)
Genetics
Protein / Probe
Kinetochore protein NDC80 homolog,
Kinetochore protein Nuf2
Method / Genotype
injection
Protein / Probe
Histone H2B type 1-J
Method / Genotype
injection
Treatments
Imaging Method
Visualization Method
Green fluorescent protein from Aequorea,
mCherry
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
Instrument
Objective
Zeiss 40×C-Apochromat 1.2NA