SSBD:database

Project

Dataset ID

Fig4G_Mut9A_6

Kind

Image data

Title

Z-series and time-lapse images of chromosomes and NDC80-deltaSPC-9A-NUF2 microbeads in a monastrol-treated and washed out oocyte

Description

Z-series and time-lapse images of chromosomes and NDC80-deltaSPC-9A-NUF2 microbeads in an oocyte. NDC80-deltaSPC-9A-NUF2 (NDC80-9A-deltaSPC and NUF2 heterodimer) beads were formed in the oocyte as follows; The authors microinjected mRNAs encoding C-terminally green fluorescent protein–tagged NDC80-9A-deltaSPC and NUF2, followed by anti-GFP–conjugated microbeads into the cytoplasm. The mRNA for H2B-mCherry was also injected to visualize the chromosomes. NDC80-9A-deltaSPC is phosphodeficient form of NDC80-deltaSPC. The imaging was conducted for 30 minutes in the presence of monastrol, and thereafter, monastrol was washed out. channel1; GFP, channel2; mCherry, channel3;bright field.

Folder Name

Fig4G_Mut9A_6

File Format

.lsm

File Size

5GB

File Separation Method

-

Figure Number

Fig4G

Dimensions

512x512x25x3x123

X Scale

0.063 micrometer

Y Scale

0.063 micrometer

Z Scale

1.25 micrometer

T Scale

5 minutes

Channel

3 channel

Quantitative Data

-

Description

Mouse oocyte which was microinjected mRNAs encoding NDC80-deltaSPC-9A-NUF2 followed by anti-GFP–conjugated microbeads (1.7 to 2.6 μm in diameter). The H2B-mCherry mRNA was also injexcted.

Organism

Strain

B6D2F1 (https://www.jax.or.jp/product-and-service/rm/detail/bdf1)

Cell

oocyte (CL:0000023)

Cell line

-

MeSH

Intrinsic Variable

-

Extrinsic Variable

Mouse oocyte was microinjected mRNAs encoding NDC80-deltaSPC-9A-NUF2 followed by anti-GFP–conjugated microbeads (1.7 to 2.6 μm in diameter). The H2B-mCherry mRNA was also injexcted. The oocyte was pretreated with monastrol and washedout during imaging.

Anatomical Entity

-

UBERON

-

Biological Process

Cellular Component

Molecular Function

-

MeSH Anatomy

MeSH BP

MeSH CC

MeSH MF

Imaging Method

Detection Method

-

Visualization Method

Green fluorescent protein from Aequorea, mCherry

Illumination Method

-

Source of Contrast

distribution of a specific protein

Imaged Parameter

fluorescence emission

Sample Preparation Method

living tissue

MeSH

Body

Zeiss LSM880

Module

-

Light Source

-

Detector

-

Objective

Zeiss 40×C-Apochromat 1.2NA

Filter Set

-

Dichroic

-