422-Asai-KinetochoreBeads/Fig4A_Mut9A_15
Author reviewed
Z-series and time-lapse images of chromosomes and NDC80-deltaSPC-9A-NUF2 microbeads in an oocyte
Title
Z-series and time-lapse images of chromosomes and NDC80-deltaSPC-9A-NUF2 microbeads in an oocyte
Description
Z-series and time-lapse images of chromosomes and NDC80-deltaSPC-9A-NUF2 microbeads in an oocyte. NDC80-deltaSPC-9A-NUF2 (NDC80-9A-deltaSPC and NUF2 heterodimer) beads were formed in the oocyte as follows; The authors microinjected mRNAs encoding
C-terminally green fluorescent protein–tagged
NDC80-9A-deltaSPC and NUF2, followed by
anti-GFP–conjugated microbeads into the cytoplasm. The mRNA for H2B-mCherry was also injected to visualize the chromosomes.
NDC80-9A-deltaSPC is phosphodeficient form of NDC80-deltaSPC.
channel1; GFP, channel2; mCherry, channel3;bright field.
Folder Name
Fig4A_Mut9A_15
Dimensions
512x512x25x3x140
Biosample
Description
Mouse oocyte which was microinjected mRNAs encoding NDC80-deltaSPC-9A-NUF2 followed by anti-GFP–conjugated microbeads (1.7 to 2.6 μm in diameter). The H2B-mCherry mRNA was also injexcted.
Extrinsic Variable
Mouse oocyte was microinjected mRNAs encoding NDC80-deltaSPC-9A-NUF2 followed by
anti-GFP–conjugated microbeads (1.7 to 2.6 μm in diameter). The H2B-mCherry mRNA was also injexcted.
Ontology
Biological Process
female meiosis chromosome segregation (
GO:0016321)
Genetics
Protein / Probe
Kinetochore protein NDC80 homolog,
Kinetochore protein Nuf2
Method / Genotype
injection
Protein / Probe
Histone H2B type 1-J
Method / Genotype
injection
Treatments
Imaging Method
Visualization Method
Green fluorescent protein from Aequorea,
mCherry
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
Instrument
Objective
Zeiss 40×C-Apochromat 1.2NA