422-Asai-KinetochoreBeads/Fig2A_prometaphase_6_lsm
Author reviewed
Z-series images of immunocytochemistry stained for NDC80-NUF2 beads, HURP, and DNA during prometaphase
Dataset ID
Fig2A_prometaphase_6_lsm
Title
Z-series images of immunocytochemistry stained for NDC80-NUF2 beads, HURP, and DNA during prometaphase
Description
Z-series images of immunocytochemistry stained for NDC80-NUF2 beads, HURP (hepatoma-upregulated protein, a kinetochore fiber marker), and DNA during prometaphase.NDC80-NUF2 (NDC80-NUF2 heterodimer) beads were formed in the oocyte as follows; The authors microinjected mRNAs encoding
C-terminally green fluorescent protein–tagged
NDC80 (NDC80-GFP) and NUF2, followed by
anti-GFP–conjugated microbeads into the cytoplasm. This .lsm dataset is the processed by Airyscan in the microscope system. The original data is the dataset; Fig2A_prometaphase_6_czi. アサセツメイヨム リョウホウトウロク
Folder Name
Fig2A_prometaphase_6_lsm
Dimensions
1000x1000x121x3x1
Biosample
Description
The fixed mouse oocyte which was microinjected mRNAs encoding
C-terminally green fluorescent protein–tagged
NDC80 (NDC80-GFP) and NUF2, followed by
anti-GFP–conjugated microbeads (1.7 to 2.6 μm in diameter), into the cytoplasm.
Extrinsic Variable
Mouse oocyte was microinjected mRNAs encoding
C-terminally green fluorescent protein–tagged
NDC80 (NDC80-GFP) and NUF2, followed by
anti-GFP–conjugated microbeads (1.7 to 2.6 μm in diameter).
Ontology
Biological Process
female meiosis chromosome segregation (
GO:0016321)
Genetics
Protein / Probe
Kinetochore protein NDC80 homolog,
Kinetochore protein Nuf2
Method / Genotype
injection
Protein / Probe
Disks large-associated protein 5 / antibody
Protein / Probe
-Hoechst 33342
Treatments
Imaging Method
Visualization Method
primary antibody plus labeled secondary antibody,
Hoechst 33342
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
fixation method
Instrument
Objective
Zeiss 40×C-Apochromat 1.2NA