SSBD:database

422-Asai-KinetochoreBeads/Fig1C_Chr-and-beads_2

Author reviewed

Z-series and time-lapse images of chromosomes and NDC80-NUF2 beads in an oocyte

SSBD:downloads

Project

422-Asai-KinetochoreBeads

Dataset ID

Fig1C_Chr-and-beads_2

Kind

Image data

Title

Z-series and time-lapse images of chromosomes and NDC80-NUF2 beads in an oocyte

Description

Z-series and time-lapse images of chromosomes and NDC80-NUF2 beads in a mouse oocyte. NDC80-NUF2 (NDC80-NUF2 heterodimer) beads were formed in the oocyte as follows; The authors microinjected mRNAs encoding C-terminally green fluorescent protein–tagged NDC80 (NDC80-GFP) and NUF2, followed by anti-GFP–conjugated microbeads into the cytoplasm. The mRNA for H2B-mCherry was also injected to visualize the chromosomes. channel1; GFP, channel2; mCherry, channel3;bright field.キジュｔウ

Folder Name

Fig1C_Chr-and-beads_2

File Format

.lsm

File Size

7.2GB

File Separation Method

Figure Number

Fig1C

Dimensions

512x512x25x3x176

X Scale

0.64 micrometer

Y Scale

0.64 micrometer

Z Scale

1.25 micrometer

T Scale

5 minutes

Channel

3 channel

Quantitative Data

Paper(s)

Biosample

Description

Mouse oocyte which was microinjected mRNAs encoding C-terminally green fluorescent protein–tagged NDC80 (NDC80-GFP) and NUF2, followed by anti-GFP–conjugated microbeads (1.7 to 2.6 μm in diameter), into the cytoplasm

Organism

Mus musculus (NCBITaxon:10090)

Strain

B6D2F1 (https://www.jax.or.jp/product-and-service/rm/detail/bdf1)

Cell

oocyte (CL:0000023)

Cell line

MeSH

Oocytes (D009865)

Microscopy, Confocal (D018613)

Meiosis (D008540)

Kinetochores (D018386)

Chromosome Segregation (D020090)

Chromosomes (D002875)

Intrinsic Variable

Extrinsic Variable

Mouse oocyte was microinjected mRNAs encoding C-terminally green fluorescent protein–tagged NDC80 (NDC80-GFP) and NUF2, followed by anti-GFP–conjugated microbeads (1.7 to 2.6 μm in diameter). The H2B-mCherry mRNA was also injexcted.

Ontology

Anatomical Entity

UBERON

Biological Process

female meiosis chromosome segregation (GO:0016321)

kinetochore assembly (GO:0051382)

Cellular Component

kinetochore (GO:0000776)

chromosome (GO:0005694)

Molecular Function

MeSH Anatomy

Oocytes (D009865)

Microscopy, Confocal (D018613)

Meiosis (D008540)

Kinetochores (D018386)

Chromosome Segregation (D020090)

Chromosomes (D002875)

MeSH BP

Oocytes (D009865)

Microscopy, Confocal (D018613)

Meiosis (D008540)

Kinetochores (D018386)

Chromosome Segregation (D020090)

Chromosomes (D002875)

MeSH CC

Oocytes (D009865)

Microscopy, Confocal (D018613)

Meiosis (D008540)

Kinetochores (D018386)

Chromosome Segregation (D020090)

Chromosomes (D002875)

MeSH MF

Oocytes (D009865)

Microscopy, Confocal (D018613)

Meiosis (D008540)

Kinetochores (D018386)

Chromosome Segregation (D020090)

Chromosomes (D002875)

Genetics

Gene

Protein / Probe

Kinetochore protein NDC80 homolog, Kinetochore protein Nuf2

Method / Genotype

injection

Gene

Protein / Probe

Histone H2B type 1-J

Method / Genotype

injection

Treatments

Reagent / Compound

Concentration

Fold Dilution

Imaging Method

confocal microscopy (FBbi:00000251)

time lapse microscopy (FBbi:00000249)

Detection Method

Visualization Method

Green fluorescent protein from Aequorea, mCherry

Illumination Method

Source of Contrast

distribution of a specific protein

Imaged Parameter

fluorescence emission

Sample Preparation Method

living tissue

MeSH

Oocytes (D009865)

Microscopy, Confocal (D018613)

Meiosis (D008540)

Kinetochores (D018386)

Chromosome Segregation (D020090)

Chromosomes (D002875)

Instrument

Body

Zeiss LSM880

Module

Light Source

Detector

Objective

Zeiss 40×C-Apochromat 1.2NA

Filter Set

Dichroic