SSBD:database

421-Takenouchi-AgedYoungOocyte/Fig5_ChrXand3_20221219_2_Aged_5

Author reviewed
Time-lapse and z-series images of H2B-SNAP and the tdTomato-CENPC in aged mouse oocyte
SSBD:downloads
Project
421-Takenouchi-AgedYoungOocyte
Dataset ID
Fig5_ChrXand3_20221219_2_Aged_5
Kind
Image data
Title
Time-lapse and z-series images of H2B-SNAP and the tdTomato-CENPC in aged mouse oocyte
Description
Time-lapse and z-series images of H2B-SNAP and the kinetochore marker tdTomato-CENPC from prometaphase to anaphase of 16 to 22 month-old-mouse oocyte. Both of xyzt-images and xyt-image (z-stacked images) were reposited.
Folder Name
Fig5_ChrXand3_20221219_2_Aged_5
File Format
.tif
File Size
1.3GB
File Separation Method
2 files
Figure Number
Fig5
Dimensions
256x256x17x3x210
X Scale
0.119 micrometer
Y Scale
0.119 micrometer
Z Scale
1.5 micrometer
T Scale
3 minutes
Channel
3 channel
Quantitative Data
-
Paper(s)

Biosample

Description
From prometaphase to anaphase oocyte from 16 to 22 month-old-mouse, in which chromosomes and kinetochores were visualized.
Organism
Mus musculus (NCBITaxon:10090)
Strain
B6D2F1/J (https://www.jax.org/strain/100006)
Cell
oocyte (CL:0000023)
Cell line
-
MeSH
Oocytes (D009865)
Microscopy, Confocal (D018613)
Meiosis (D008540)
Chromosomes (D002875)
Chromosome Segregation (D020090)
Intrinsic Variable
-
Extrinsic Variable
To visualize chromosomes and kinetochores, in vitro transcribed mRNAs for H2B-SNAP and tdTomato-CENPC (centromere protein C) were injected. To detect chromosomeX and chromosome3, MCP-3tagBFP and PCP-3mClover were also introduced for CRISPR-Sirius method.

Ontology

Anatomical Entity
-
UBERON
oocyte (CL:0000023)
Biological Process
female meiosis chromosome segregation, (GO:0016321)
Cellular Component
chromosome (GO:0005694)
Molecular Function
-
MeSH Anatomy
Oocytes (D009865)
Microscopy, Confocal (D018613)
Meiosis (D008540)
Chromosomes (D002875)
Chromosome Segregation (D020090)
MeSH BP
Oocytes (D009865)
Microscopy, Confocal (D018613)
Meiosis (D008540)
Chromosomes (D002875)
Chromosome Segregation (D020090)
MeSH CC
Oocytes (D009865)
Microscopy, Confocal (D018613)
Meiosis (D008540)
Chromosomes (D002875)
Chromosome Segregation (D020090)
MeSH MF
Oocytes (D009865)
Microscopy, Confocal (D018613)
Meiosis (D008540)
Chromosomes (D002875)
Chromosome Segregation (D020090)

Genetics

Gene
-
Protein / Probe
Histone H2B type 1-J
Method / Genotype
injection
Gene
-
Protein / Probe
Centromere protein C
Method / Genotype
injection

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy (FBbi:00000251)
time lapse microscopy (FBbi:00000249)
Detection Method
photomultiplier tube (PMT)
Visualization Method
H2B with SNAP-tag was visuallised with SNAP-Cell 648-SiR. To visualize kinetochores, centromere protein C was tagged with tdTomato.
Illumination Method
single point scanning
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Oocytes (D009865)
Microscopy, Confocal (D018613)
Meiosis (D008540)
Chromosomes (D002875)
Chromosome Segregation (D020090)

Instrument

Body
Zeiss LSM780
Module
-
Light Source
-
Detector
-
Objective
Zeiss 40×C-Apochromat 1.2NA
Filter Set
-
Dichroic
-