SSBD:database

421-Takenouchi-AgedYoungOocyte/Fig1D_ChrXand3_8

Author reviewed

Time-lapse and z-series images of H2B-SNAP and the tdTomato-CENPC in young mouse oocyte

SSBD:downloads

Project

421-Takenouchi-AgedYoungOocyte

Dataset ID

Fig1D_ChrXand3_8

Kind

Image data

Title

Time-lapse and z-series images of H2B-SNAP and the tdTomato-CENPC in young mouse oocyte

Description

Time-lapse and z-series images of H2B-SNAP and the kinetochore marker tdTomato-CENPC from prometaphase to anaphase of 7 to 9 week-old-mouse oocyte. The position of chromosomeX and chromosome3 at a certain time during imaging, which were detected with their specific probe, were indicated in the .xls file in this project. channel1; H2B-SNAP, channel2; dTomato-CENPC, channel3; bright field

Folder Name

Fig1D_ChrXand3_8

File Format

tif

File Size

1.4GB

File Separation Method

Figure Number

Fig1D

Dimensions

256x256x17x3x220

X Scale

0.119 micrometer

Y Scale

0.119 micrometer

Z Scale

1.5 micrometer

T Scale

3 minutes

Channel

3 channel

Quantitative Data

Paper(s)

Biosample

Description

From prometaphase to anaphase oocyte from 7 to 9 week-old-mouse, in which chromosomes and kinetochores were visualized.

Organism

Mus musculus (NCBITaxon:10090)

Strain

B6D2F1/J (https://www.jax.org/strain/100006)

Cell

oocyte (CL:0000023)

Cell line

MeSH

Oocytes (D009865)

Microscopy, Confocal (D018613)

Meiosis (D008540)

Chromosomes (D002875)

Chromosome Segregation (D020090)

Intrinsic Variable

Extrinsic Variable

To visualize chromosomes and kinetochores, in vitro transcribed mRNAs for H2B-SNAP and tdTomato-CENPC (centromere protein C) were injected. To detect chromosomeX and chromosome3, MCP-3tagBFP and PCP-3mClover were also introduced for CRISPR-Sirius method.

Ontology

Anatomical Entity

UBERON

oocyte (CL:0000023)

Biological Process

female meiosis chromosome segregation, (GO:0016321)

Cellular Component

chromosome (GO:0005694)

Molecular Function

MeSH Anatomy

Oocytes (D009865)

Microscopy, Confocal (D018613)

Meiosis (D008540)

Chromosomes (D002875)

Chromosome Segregation (D020090)

MeSH BP

Oocytes (D009865)

Microscopy, Confocal (D018613)

Meiosis (D008540)

Chromosomes (D002875)

Chromosome Segregation (D020090)

MeSH CC

Oocytes (D009865)

Microscopy, Confocal (D018613)

Meiosis (D008540)

Chromosomes (D002875)

Chromosome Segregation (D020090)

MeSH MF

Oocytes (D009865)

Microscopy, Confocal (D018613)

Meiosis (D008540)

Chromosomes (D002875)

Chromosome Segregation (D020090)

Genetics

Gene

Protein / Probe

Histone H2B type 1-J

Method / Genotype

injection

Gene

Protein / Probe

Centromere protein C

Method / Genotype

injection

Treatments

Reagent / Compound

Concentration

Fold Dilution

Imaging Method

confocal microscopy (FBbi:00000251)

time lapse microscopy (FBbi:00000249)

Detection Method

photomultiplier tube (PMT)

Visualization Method

H2B with SNAP-tag was visuallised with SNAP-Cell 647-SiR. To visualize kinetochores, centromere protein C was tagged with tdTomato.

Illumination Method

single point scanning

Source of Contrast

distribution of a specific protein

Imaged Parameter

fluorescence emission

Sample Preparation Method

living tissue

MeSH

Oocytes (D009865)

Microscopy, Confocal (D018613)

Meiosis (D008540)

Chromosomes (D002875)

Chromosome Segregation (D020090)

Instrument

Body

Zeiss LSM780

Module

Light Source

Detector

Objective

Zeiss 40×C-Apochromat 1.2NA

Filter Set

Dichroic