SSBD:database

419-Hagiwara-CollectiveMigrate/Fig3e_Lami_FN

Author reviewed
Z-series images of immunostaining of laminin and fibronectin after collective cell migration into matrigel
SSBD:downloads
Project
419-Hagiwara-CollectiveMigrate
Dataset ID
Fig3e_Lami_FN
Kind
Image data
Title
Z-series images of immunostaining of laminin and fibronectin after collective cell migration into matrigel
Description
Z-series images of immunostaining of laminin and fibronectin after collective cell migration into matrigel.
Folder Name
Fig3e_Lami_FN
File Format
.lif
File Size
82MB
File Separation Method
-
Figure Number
Fig3e
Dimensions
512x512x95x3x1
X Scale
0.278 micrometer
Y Scale
0.278 micrometer
Z Scale
0.335 micrometer
T Scale
-
Channel
3 channel
Quantitative Data
-
Paper(s)

Biosample

Description
NHBE (human bronchial epithelial) cells coverd with growth factor-reduced matrigel.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
-
Cell line
NHBE cell (BTO:0002924)
MeSH
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell Movement (D002465)
Cell-Matrix Junctions (D021982)
Microscopy, Confocal (D018613)
Extracellular Matrix (D005109)
Intrinsic Variable
-
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
cell (CL:0000000)
Biological Process
epithelial cell migration (GO:0010631)
cell-matrix recognition (GO:0009989)
Cellular Component
cell-substrate junction (GO:0030055)
extracellular matrix (GO:0031012)
Molecular Function
-
MeSH Anatomy
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell Movement (D002465)
Cell-Matrix Junctions (D021982)
Microscopy, Confocal (D018613)
Extracellular Matrix (D005109)
MeSH BP
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell Movement (D002465)
Cell-Matrix Junctions (D021982)
Microscopy, Confocal (D018613)
Extracellular Matrix (D005109)
MeSH CC
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell Movement (D002465)
Cell-Matrix Junctions (D021982)
Microscopy, Confocal (D018613)
Extracellular Matrix (D005109)
MeSH MF
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell Movement (D002465)
Cell-Matrix Junctions (D021982)
Microscopy, Confocal (D018613)
Extracellular Matrix (D005109)

Genetics

Gene
-
Protein / Probe
Laminin subunit alpha-1 / antibody
Method / Genotype
-
Gene
-
Protein / Probe
Fibronectin / antibody
Method / Genotype
-
Gene
-
Protein / Probe
-4',6-diamidino-2-phenylindole (DAPI)
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
fluorescence microscopy (FBbi:00000246)
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
primary antibody plus labeled secondary antibody
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
fixation method
MeSH
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell Movement (D002465)
Cell-Matrix Junctions (D021982)
Microscopy, Confocal (D018613)
Extracellular Matrix (D005109)

Instrument

Body
Leica SP8 lightning confocal microscope
Module
-
Light Source
-
Detector
-
Objective
-
Filter Set
-
Dichroic
-