SSBD:database

419-Hagiwara-CollectiveMigrate/Fig2g_ActinNucleus

Author reviewed
Z-series immunostaining images of NHEB cells cultured with or without matrigel cover
SSBD:downloads
Project
419-Hagiwara-CollectiveMigrate
Dataset ID
Fig2g_ActinNucleus
Kind
Image data
Title
Z-series immunostaining images of NHEB cells cultured with or without matrigel cover
Description
Z-series immunostaining images of NHEB cells cultured with (0h, 24h, 48h) or without (24h) matrigel cover. The actin and nucleus were immunostained.
Folder Name
Fig2g_ActinNucleus
File Format
.lif
File Size
70MB, 142MB, 53MB, 44MB
File Separation Method
The data of different 4 conditions are included.
Figure Number
Fig2g
Dimensions
512x512x140x2x1 512x512x284x2x1, 512x512x106x2x1 512x512x88x2x1
X Scale
0.181 micrometer
Y Scale
0.181 micrometer
Z Scale
0.335 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
NHBE (human bronchial epithelial) cells coverd with or without growth factor-reduced matrigel.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
-
Cell line
NHBE cell (BTO:0002924)
MeSH
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell Shape (D048430)
Actin Cytoskeleton (D008841)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
Intrinsic Variable
-
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
cell (CL:0000000)
Biological Process
regulation of cell shape (GO:0008360)
Cellular Component
actin cytoskeleton (GO:0015629)
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell Shape (D048430)
Actin Cytoskeleton (D008841)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
MeSH BP
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell Shape (D048430)
Actin Cytoskeleton (D008841)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
MeSH CC
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell Shape (D048430)
Actin Cytoskeleton (D008841)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
MeSH MF
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell Shape (D048430)
Actin Cytoskeleton (D008841)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)

Genetics

Gene
-
Protein / Probe
Actin, cytoplasmic 1 / antibody
Method / Genotype
-
Gene
-
Protein / Probe
-4',6-diamidino-2-phenylindole (DAPI)
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
fluorescence microscopy (FBbi:00000246)
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
primary antibody plus labeled secondary antibody
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
fixation method
MeSH
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell Shape (D048430)
Actin Cytoskeleton (D008841)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)

Instrument

Body
Leica SP8 lightning confocal microscope
Module
-
Light Source
-
Detector
-
Objective
-
Filter Set
-
Dichroic
-