SSBD:database

419-Hagiwara-CollectiveMigrate/Fig2b_BasalApical

Author reviewed
Z-series images of Integrin beta4 and Crb3 immunostaining in NHBE cells cultured without matrigel cover
SSBD:downloads
Project
419-Hagiwara-CollectiveMigrate
Dataset ID
Fig2b_BasalApical
Kind
Image data
Title
Z-series images of Integrin beta4 and Crb3 immunostaining in NHBE cells cultured without matrigel cover
Description
Z-series images of Integrin beta4 and Crb3 immunostaining in NHBE cells cultured without matrigel cover. The cells were fixed and stained 3 days after the cell seeding.
Folder Name
Fig2b_BasalApical
File Format
.lifext
File Size
6.4MB
File Separation Method
-
Figure Number
Fig2b
Dimensions
256x256x29x2x1, 93x93x14x2x1
X Scale
0.181 micrometer
Y Scale
0.181 micrometer
Z Scale
0.335 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
The fixed NHBE (human bronchial epithelial) cells 3 days after seeding.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
-
Cell line
NHBE cell (BTO:0002924)
MeSH
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell-Matrix Junctions (D021982)
Microscopy, Confocal (D018613)
Intrinsic Variable
-
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
cell (CL:0000000)
Biological Process
-
Cellular Component
cell-substrate junction (GO:0030055)
plasma membrane (GO:0005886)
Molecular Function
-
MeSH Anatomy
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell-Matrix Junctions (D021982)
Microscopy, Confocal (D018613)
MeSH BP
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell-Matrix Junctions (D021982)
Microscopy, Confocal (D018613)
MeSH CC
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell-Matrix Junctions (D021982)
Microscopy, Confocal (D018613)
MeSH MF
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell-Matrix Junctions (D021982)
Microscopy, Confocal (D018613)

Genetics

Gene
-
Protein / Probe
Integrin beta-4 / antibody
Method / Genotype
-
Gene
-
Protein / Probe
Protein crumbs homolog 3 / antibody
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
fluorescence microscopy (FBbi:00000246)
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
primary antibody plus labeled secondary antibody
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
dispersed cells in vitro
MeSH
Microscopy, Fluorescence (D008856)
Cells (D002477)
Cell-Matrix Junctions (D021982)
Microscopy, Confocal (D018613)

Instrument

Body
Leica SP8 lightning confocal microscope
Module
-
Light Source
-
Detector
-
Objective
-
Filter Set
-
Dichroic
-