416-Otomo-descSPIM/Fig4_xenograft_plus1000_1
Author reviewedZ-series images of a BT-474 cell line-derived xenograft labeled with DyLight 650-Trastuzumab, PI, and CD31
Project
Dataset ID
Fig4_xenograft_plus1000_1
Kind
Image data
Title
Z-series images of a BT-474 cell line-derived xenograft labeled with DyLight 650-Trastuzumab, PI, and CD31
Description
Z-series images of a xenograft derived from BT-474 human breast cancer cell line. The tumor xenograft was labeled with DyLight 650-Trastuzumab, propidium iodide, and CD31. The imaging was conducted with descSPIM, the desktop- equipped SPIM (selective plane illumination microscopy). The light sheet focus is 1000 um left side of the xenograft along the X axis.
Folder Name
Fig4_xenograft_plus1000_1
File Format
.tif
File Size
18.1GB
File Separation Method
each channel is separated to individual files
Figure Number
Fig4
Dimensions
2160x4096x681x3x1
X Scale
3.5 micrometer
Y Scale
3.5 micrometer
Z Scale
10 micrometer
T Scale
-
Channel
3 channel
Quantitative Data
-
Paper(s)
Biosample
Description
BT-474 human breast cancer cells suspended in PBS and equal volume of Matrigel was subcutaneously
injected into the flank of the 5 to 6-week-old female SCID-beige mouse. FITC-labeled anti-murine CD31 antibody and the DyLightTM 650-labeled Trastuzumab were
intravenously administered from the tail vein 24 h before sacrifice. The xenograft was excised, fixed, cleared, and stained.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
-
Cell line
BT-474 cell (CLO:0002042)
Intrinsic Variable
-
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
nucleus (GO:0005634)
plasma membrane (GO:0005886)
Molecular Function
-
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
-
Gene
-
Protein / Probe
-propidium iodide
Method / Genotype
-
Gene
-
Protein / Probe
Platelet endothelial cell adhesion molecule / labeled primary antibody
Method / Genotype
-
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
fluorescence microscopy (FBbi:00000246)
selective plane illumination microscopy (SPIM)
Detection Method
-
Visualization Method
propidium iodide,
DyLight 650,
FITC
Illumination Method
light-sheet illumination
Source of Contrast
distribution of a specific protein,
compartmentalization of stain or label
Imaged Parameter
fluorescence emission
Sample Preparation Method
fixation method
Instrument
Body
custom made
Module
-
Light Source
Cobolt Skyra 488 nm, 561 nm and 647 nm
Detector
Thorlabs CMOS camera CS895MU
Objective
Thorlabs TL2X-SAP (NA = 0.1, WD= 56.3mm)
Filter Set
Thorlabs, FBH520-40, FBH600-40 and FBH700-40
Dichroic
-