SSBD:database

412-Katoh-BMP4Cilia/Fig4C_BeforeBend

Author reviewed

Z-series calcium imaging of immotile cilia and crown cells of Inversin mutant embryo in the presence of Noggin before manipulation of cilia using optical tweezer

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Project

412-Katoh-BMP4Cilia

Dataset ID

Fig4C_BeforeBend

Kind

Image data

Title

Z-series calcium imaging of immotile cilia and crown cells of Inversin mutant embryo in the presence of Noggin before manipulation of cilia using optical tweezer

Description

Z-series calcium imaging of immotile cilia and crown cells of iv/iv mouse embryo in the presence of Noggin before manipulation of cilia using optical tweezer. GCaMP6 was expressed in the cytoplasm for cytoplasmic calcium imaging. Immotile cilia at the node were visualized based on mCherry expression. The images of mCherry were partially obtained from T=2 to T= 66 of calcium images.

Folder Name

Fig4C_BeforeBend

File Format

.tif

File Size

2.2GB, 1GB

File Separation Method

2 channels are separated into two files

Figure Number

Fig4C

Dimensions

512x512x30x1x150, 512x512x30x1x66

X Scale

0.43 micrometer

Y Scale

0.43 micrometer

Z Scale

1 micrometer

T Scale

6.484 seconds

Channel

2 channel

Quantitative Data

Paper(s)

Biosample

Description

iv/iv mouse embryo harboring NDE4–hsp– 5HT6–GCaMP6–2A–5HT6–mCherry and NDE4–hsp– GCaMP6

Organism

Mus musculus

(NCBITaxon:10090)

Strain

Cell

crown cell

Cell line

MeSH

Time-Lapse Imaging (D059008)

Embryo, Mammalian (D004622)

Calcium Signaling (D020013)

Intrinsic Variable

NDE4–hsp– 5HT6–GCaMP6–2A–5HT6–mCherry and NDE4–hsp– GCaMP6

Extrinsic Variable

Reconbinant Noggin was treated from late bud stage to 0-2 somite stages.

Ontology

Anatomical Entity

UBERON

embryo (UBERON:0000922)

Biological Process

calcium-mediated signaling (GO:0019722)

Cellular Component

non-motile cilium (GO:0097730)

Molecular Function

MeSH Anatomy

Time-Lapse Imaging (D059008)

Embryo, Mammalian (D004622)

Calcium Signaling (D020013)

MeSH BP

Time-Lapse Imaging (D059008)

Embryo, Mammalian (D004622)

Calcium Signaling (D020013)

MeSH CC

Time-Lapse Imaging (D059008)

Embryo, Mammalian (D004622)

Calcium Signaling (D020013)

MeSH MF

Time-Lapse Imaging (D059008)

Embryo, Mammalian (D004622)

Calcium Signaling (D020013)

Genetics

Gene

Protein / Probe

Method / Genotype

transgenic / Dnah11 tm1Bknr, B6-Tg(Nodal NDE-5HT6/GCaMP6,5HT6/mCherry)35Hmd, STOCK Tg(Nodal NDE-GCaMP6)29AHmd

Gene

Protein / Probe

Method / Genotype

transgenic / Dnah11 tm1Bknr, B6-Tg(Nodal NDE-5HT6/GCaMP6,5HT6/mCherry)35Hmd, STOCK Tg(Nodal NDE-GCaMP6)29AHmd

Gene

Dnah11

Protein / Probe

Method / Genotype

gene knock out / Dnah11 tm1Bknr, B6-Tg(Nodal NDE-5HT6/GCaMP6,5HT6/mCherry)35Hmd, STOCK Tg(Nodal NDE-GCaMP6)29AHmd

Treatments

Reagent / Compound

Noggin

Concentration

1 microgram per milliliter

Fold Dilution

Imaging Method

time lapse microscopy (FBbi:00000249)

Detection Method

Visualization Method

GCaMP6

Illumination Method

Source of Contrast

Imaged Parameter

fluorescence emission

Sample Preparation Method

living tissue

MeSH

Time-Lapse Imaging (D059008)

Embryo, Mammalian (D004622)

Calcium Signaling (D020013)

Instrument

Body

Olympus-Evident IX83

Module

Light Source

Detector

Objective

Olympus-Evident UPLSAPO60XW

Filter Set

Dichroic