412-Katoh-BMP4Cilia/Fig2E_InversinMut
Author reviewedZ-series STED images of immotile cilia in Inversin mutant embryo
Project
Dataset ID
Fig2E_InversinMut
Kind
Image data
Title
Z-series STED images of immotile cilia in Inversin mutant embryo
Description
Z-series STED images of immotile cilia in Inversin mutant embryo. The immotile cilia in mouse embryo at 0-2 somite stages were immunostained with Pkd2 and acetylated tubulin.
Folder Name
Fig2E_InversinMut
File Format
.tif
File Size
1.7GB
File Separation Method
-
Figure Number
Fig2E
Dimensions
30x95x77x2x1
X Scale
0.03 micrometer
Y Scale
0.03 micrometer
Z Scale
0.04 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
A cultured Inversin mutant mouse embryo from the late bud stage, just before the beginning of node formation, to the 0-2 somite stages,
Organism
Mus musculus
Strain
-
Cell
-
Cell line
-
MeSH
Embryo, Mammalian (D004622)
Intrinsic Variable
Inversin mutant mouse
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
embryo (UBERON:0000922)
Biological Process
-
Cellular Component
non-motile cilium (GO:0097730)
Molecular Function
-
MeSH Anatomy
Embryo, Mammalian (D004622)
MeSH BP
Embryo, Mammalian (D004622)
MeSH CC
Embryo, Mammalian (D004622)
MeSH MF
Embryo, Mammalian (D004622)
Genetics
Gene
-
Protein / Probe
Polycystin-2 / antibody
Method / Genotype
-FVB-Invs<inv>(CCM)
Gene
-
Protein / Probe
Tubulin alpha-1A chain / antibody
Method / Genotype
-FVB-Invs<inv>(CCM)
Gene
Invs
Protein / Probe
-
Method / Genotype
gene knock out / FVB-Invs<inv>(CCM)
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
stimulated emission depletion (STED) (FBbi:00000334)
Detection Method
-
Visualization Method
primary antibody plus labeled secondary antibody
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
fixation method
MeSH
Embryo, Mammalian (D004622)
Instrument
Body
abberior infinity
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident UPLXAPO60XO
Filter Set
-
Dichroic
-