412-Katoh-BMP4Cilia/Fig1I_SU5402
Author reviewedZ-series images of immotile cilia with SU5402 treatment
Project
Dataset ID
Fig1I_SU5402
Kind
Image data
Title
Z-series images of immotile cilia with SU5402 treatment
Description
Z-series images of immotile cilia with SU5402 (an inhibitor of the tyrosine kinase activity of FGF and vascular endothelial growth factor receptors) treatment from late bud stage of the cultured embryo. The immotile cilia in mouse embryo at 0-2 somite stages were immunostained with Pkd2::Venus and acetylated tubulin.
Folder Name
Fig1I_SU5402
File Format
.czi
File Size
2.2GB
File Separation Method
-
Figure Number
Fig1I
Dimensions
2172x2172x123x2x1
X Scale
0.04 micrometer
Y Scale
0.04 micrometer
Z Scale
0.168 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
A cultured mouse embryo from the late bud stage, just before the beginning of node formation, to the 0-2 somite stages,
Organism
Mus musculus
Strain
-
Cell
-
Cell line
-
Intrinsic Variable
NDE2–hsp–Pkd2–Venus mouse, in whoch Pkd2–Venus is expressed by the nodal-specific
enhancer (NDE)
Extrinsic Variable
SU5402 was treated from late bud stage to 0-2 somite stages.
Ontology
Anatomical Entity
-
UBERON
embryo (UBERON:0000922)
Biological Process
-
Cellular Component
non-motile cilium (GO:0097730)
Molecular Function
-
Genetics
Gene
-
Protein / Probe
Polycystin-2
Method / Genotype
transgenic / B6;B6C3(129)-Tg(NDE-hsp-Pkd2/Venus)1Hmd
Gene
-
Protein / Probe
Tubulin alpha-1A chain / antibody
Method / Genotype
-B6;B6C3(129)-Tg(NDE-hsp-Pkd2/Venus)1Hmd
Treatments
Reagent / Compound
SU5402
Concentration
25 micromolar
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
primary antibody plus labeled secondary antibody
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
fixation method
Instrument
Body
Zeiss LSM 880
Module
-
Light Source
-
Detector
-
Objective
Zeiss alpha Plan-Apochromat 100x/1.46 Oil
Filter Set
-
Dichroic
-