412-Katoh-BMP4Cilia/Fig1G_DAPT
Author reviewedZ-series images of immotile cilia with DAPT treatment
Project
Dataset ID
Fig1G_DAPT
Kind
Image data
Title
Z-series images of immotile cilia with DAPT treatment
Description
Z-series images of immotile cilia with DAPT ( N-[N-(3, 5-difluorophenacetyl)-l-alanyl]-S- phenylglycine t-butyl ester) treatment from late bud stage of the cultured embryo. The immotile cilia in mouse embryo at 0-2 somite stages were immunostained with Pkd2::Venus and acetylated tubulin.
Folder Name
Fig1G_DAPT
File Format
.czi
File Size
2.1GB
File Separation Method
-
Figure Number
Fig1G
Dimensions
2172x2172x120x2x1
X Scale
0.04 micrometer
Y Scale
0.04 micrometer
Z Scale
0.168 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
A cultured mouse embryo from the late bud stage, just before the beginning of node formation, to the 0-2 somite stages,
Organism
Mus musculus
Strain
-
Cell
-
Cell line
-
Intrinsic Variable
NDE2–hsp–Pkd2–Venus mouse, in whoch Pkd2–Venus is expressed by the nodal-specific
enhancer (NDE)
Extrinsic Variable
DAPT was treated from late bud stage to 0-2 somite stages.
Ontology
Anatomical Entity
-
UBERON
embryo (UBERON:0000922)
Biological Process
-
Cellular Component
non-motile cilium (GO:0097730)
Molecular Function
-
Genetics
Gene
-
Protein / Probe
Polycystin-2
Method / Genotype
transgenic / B6;B6C3(129)-Tg(NDE-hsp-Pkd2/Venus)1Hmd
Gene
-
Protein / Probe
Tubulin alpha-1A chain / antibody
Method / Genotype
-B6;B6C3(129)-Tg(NDE-hsp-Pkd2/Venus)1Hmd
Treatments
Reagent / Compound
DAPT
Concentration
200 micromolar
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
primary antibody plus labeled secondary antibody
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
fixation method
Instrument
Body
Zeiss LSM 880
Module
-
Light Source
-
Detector
-
Objective
Zeiss alpha Plan-Apochromat 100x/1.46 Oil
Filter Set
-
Dichroic
-