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412-Katoh-BMP4Cilia/Fig1E_Smo thumbnail

412-Katoh-BMP4Cilia/Fig1E_Smo

Author reviewed
Z-series images of immotile cilia in Smo mutant embryo
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Project
412-Katoh-BMP4Cilia
Dataset ID
Fig1E_Smo
Kind
Image data
Title
Z-series images of immotile cilia in Smo mutant embryo
Description
Z-series images of immotile cilia in Smo, which encodes a G protein-coupled receptor, mutant embryo. The immotile cilia in mouse embryo at 0-2 somite stages were immunostained with Pkd2::Venus and acetylated tubulin.
Folder Name
Fig1E_Smo
File Format
.czi
File Size
3.6GB
File Separation Method
-
Figure Number
Fig1E
Dimensions
2172x2172x173x2x1
X Scale
0.04 micrometer
Y Scale
0.04 micrometer
Z Scale
0.168 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
A cultured mouse embryo from the late bud stage, just before the beginning of node formation, to the 0-2 somite stages,
Organism
Mus musculus
(NCBITaxon:10090)
Strain
-
Cell
-
Cell line
-
MeSH
Microscopy, Confocal (D018613)
Embryo, Mammalian (D004622)
Intrinsic Variable
NDE2–hsp–Pkd2–Venus mouse, in whoch Pkd2–Venus is expressed by the nodal-specific enhancer (NDE). Mutant mouse lacking Smo.
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
embryo (UBERON:0000922)
Biological Process
-
Cellular Component
non-motile cilium (GO:0097730)
Molecular Function
-
MeSH Anatomy
Microscopy, Confocal (D018613)
Embryo, Mammalian (D004622)
MeSH BP
Microscopy, Confocal (D018613)
Embryo, Mammalian (D004622)
MeSH CC
Microscopy, Confocal (D018613)
Embryo, Mammalian (D004622)
MeSH MF
Microscopy, Confocal (D018613)
Embryo, Mammalian (D004622)

Genetics

Gene
-
Protein / Probe
Polycystin-2
Method / Genotype
transgenic / B6;B6C3(129)-Tg(NDE-hsp-Pkd2/Venus)1Hmd, B6(D2)-Smo<Rgsc2073>)
Gene
-
Protein / Probe
Tubulin alpha-1A chain / antibody
Method / Genotype
-B6;B6C3(129)-Tg(NDE-hsp-Pkd2/Venus)1Hmd, B6(D2)-Smo<Rgsc2073>)
Gene
Smo
Protein / Probe
-
Method / Genotype
transgenic / B6;B6C3(129)-Tg(NDE-hsp-Pkd2/Venus)1Hmd, B6(D2)-Smo<Rgsc2073>)

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
primary antibody plus labeled secondary antibody
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
fixation method
MeSH
Microscopy, Confocal (D018613)
Embryo, Mammalian (D004622)

Instrument

Body
Zeiss LSM 880
Module
-
Light Source
-
Detector
-
Objective
Zeiss alpha Plan-Apochromat 100x/1.46 Oil
Filter Set
-
Dichroic
-