411-Yoshinari-BorateTransporter/Fig6A_BOR1_3D-SCLIM
Author reviewedZ-series and time-lapse SCLIM images of BOR1-GFP and AP4M-TagRFP in the root epidermal cells after treatment with high concentration of boric acid
Dataset ID
Fig6A_BOR1_3D-SCLIM
Kind
Image data
Title
Z-series and time-lapse SCLIM images of BOR1-GFP and AP4M-TagRFP in the root epidermal cells after treatment with high concentration of boric acid
Description
Z-series and time-lapse SCLIM (super-resolution confocal live-imaging microscopy) images of BOR1(borate transporter)-GFP and AP4M-TagRFP in the root epidermal cells after treatment with high (100 micromolar) concentration of boric acid. The images are also included that were processed using deconvolution algorithms with theoretical point-spread functions
in Volocity software.
Folder Name
Fig6A_BOR1_3D-SCLIM
File Format
.tif
File Size
10MB,
21MB
File Separation Method
pre, post
Figure Number
Fig6A
Dimensions
160x190x9x2x10,
160x190x9x2x10
X Scale
-
Y Scale
-
Z Scale
-
T Scale
4 seconds
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
The roots of Arabidopsis thaliana grown on MGRL medium contain-
ing 0.5 micromolar boric acid, 1.5% gellan gum and 1% sucrose for 4 or 5 days.
Organism
Arabidopsis thaliana (NCBITaxon:3702)
Strain
Cell
epidermal cell (CL:0000362)
Cell line
-
MeSH
Intrinsic Variable
-
Extrinsic Variable
BOR1-GFP and AP4M-TagRFP were exogenously expressed.
A. thaliana roots were treated with 100 micromolar boric acid at the start of imaging.
Ontology
Anatomical Entity
root (PO:0009005)
root epidermis (PO:0006036)
UBERON
-
Biological Process
vesicle-mediated transport (GO:0016192)
intracellular borate homeostasis (GO:0160070)
Cellular Component
Molecular Function
active borate transmembrane transporter activity (GO:0046715)
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
Boron transporter 1
Method / Genotype
binary vector method
Gene
-
Protein / Probe
AP-4 complex subunit mu
Method / Genotype
Agrobacterium-mediated floral-dip transformation
Treatments
Reagent / Compound
boric acid
Concentration
100 micromolar
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
Green fluorescent protein from Aequorea
TagRFP
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Olympus-Evident IX73
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident UPLSAPO100XS
Filter Set
-
Dichroic
-