SSBD:database

411-Yoshinari-BorateTransporter/Fig5D_BOR1_SCLIM thumbnail

411-Yoshinari-BorateTransporter/Fig5D_BOR1_SCLIM

Author reviewed
Z-series images of BOR1-GFP, AP1M2-TagRFP and AP4M-iRFP in Arabidopsis root obtained by SCLIM
SSBD:OMERO.gallery (Project) SSBD:OMERO (Project) SSBD:OMERO.gallery (Dataset) SSBD:OMERO (Dataset)
SSBD:downloads
Project
411-Yoshinari-BorateTransporter
Dataset ID
Fig5D_BOR1_SCLIM
Kind
Image data
Title
Z-series images of BOR1-GFP, AP1M2-TagRFP and AP4M-iRFP in Arabidopsis root obtained by SCLIM
Description
Z-series images of BOR1(borate transporter)-GFP, AP1M2-TagRFP and AP4M-iRFP in Arabidopsis root obtained by SCLIM (super-resolution confocal live-imaging microscopy). The images are also included that were processed using deconvolution algorithms with theoretical point-spread functions in Volocity software.
Folder Name
Fig5D_BOR1_SCLIM
File Format
.tif
File Size
37MB, 74MB
File Separation Method
pre, post
Figure Number
Fig5D
Dimensions
300x300x72x3x1, 300x300x72x3x1
X Scale
-
Y Scale
-
Z Scale
-
T Scale
-
Channel
3 channel
Quantitative Data
-
Paper(s)

Biosample

Description
The roots of Arabidopsis thaliana grown on MGRL medium contain- ing 0.5 micromolar boric acid, 1.5% gellan gum and 1% sucrose for 4 or 5 days.
Organism
Arabidopsis thaliana (NCBITaxon:3702)
Strain
Col-0 (https://arapheno.1001genomes.org/accession/6909/)
Cell
epidermal cell (CL:0000362)
Cell line
-
MeSH
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Plant Roots (D018517)
Protein Transport (D021381)
Intracellular Membranes (D007425)
Biological Transport (D001692)
Cell Membrane (D002462)
Vacuoles (D014617)
Intrinsic Variable
-
Extrinsic Variable
BOR1-GFP, AP1M2-TagRFP and AP4M-iRFP were exogenously expressed. A. thaliana roots were treated with 100 micromolar boric acid at the start of imaging.

Ontology

Anatomical Entity
root (PO:0009005)
root epidermis (PO:0006036)
UBERON
-
Biological Process
vesicle-mediated transport (GO:0016192)
intracellular borate homeostasis (GO:0160070)
Cellular Component
vesicle membrane (GO:0012506)
plasma membrane (GO:0005886)
vacuole (GO:0005773)
Molecular Function
active borate transmembrane transporter activity (GO:0046715)
MeSH Anatomy
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Plant Roots (D018517)
Protein Transport (D021381)
Intracellular Membranes (D007425)
Biological Transport (D001692)
Cell Membrane (D002462)
Vacuoles (D014617)
MeSH BP
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Plant Roots (D018517)
Protein Transport (D021381)
Intracellular Membranes (D007425)
Biological Transport (D001692)
Cell Membrane (D002462)
Vacuoles (D014617)
MeSH CC
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Plant Roots (D018517)
Protein Transport (D021381)
Intracellular Membranes (D007425)
Biological Transport (D001692)
Cell Membrane (D002462)
Vacuoles (D014617)
MeSH MF
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Plant Roots (D018517)
Protein Transport (D021381)
Intracellular Membranes (D007425)
Biological Transport (D001692)
Cell Membrane (D002462)
Vacuoles (D014617)

Genetics

Gene
-
Protein / Probe
Boron transporter 1
Method / Genotype
binary vector method
Gene
-
Protein / Probe
AP-1 complex subunit mu-2
Method / Genotype
Agrobacterium-mediated floral-dip transformation
Gene
-
Protein / Probe
AP-4 complex subunit mu
Method / Genotype
Agrobacterium-mediated floral-dip transformation

Treatments

Reagent / Compound
boric acid
Concentration
100 micromolar
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
Green fluorescent protein from Aequorea TagRFP iRFP713
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Plant Roots (D018517)
Protein Transport (D021381)
Intracellular Membranes (D007425)
Biological Transport (D001692)
Cell Membrane (D002462)
Vacuoles (D014617)

Instrument

Body
Olympus-Evident IX73
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident UPLSAPO100XS
Filter Set
-
Dichroic
-