SSBD:database

411-Yoshinari-BorateTransporter/Fig2A_BOR1_ap4b-2 thumbnail

411-Yoshinari-BorateTransporter/Fig2A_BOR1_ap4b-2

Author reviewed
Time-series images of BOR1-GFP in the root epidermis of ap4b-2 mutant Arabidopsis after high borate application
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Project
411-Yoshinari-BorateTransporter
Dataset ID
Fig2A_BOR1_ap4b-2
Kind
Image data
Title
Time-series images of BOR1-GFP in the root epidermis of ap4b-2 mutant Arabidopsis after high borate application
Description
Time-series images of BOR1 (borate transporter)-GFP and FM4-64 in the root epidermis of ap4b-2 mutant Arabidopsis after high borate (100 micromolar) application. The images of 3, 15, 30, 45, 60, 90, 120 minutes after borate applocation are included.
Folder Name
Fig2A_BOR1_ap4b-2
File Format
.czi
File Size
21MB
File Separation Method
-
Figure Number
Fig2A
Dimensions
1024x1024x1x2x7
X Scale
0.156 micrometer
Y Scale
0.156 micrometer
Z Scale
-
T Scale
15 minutes
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
The roots of Arabidopsis thaliana grown on MGRL medium contain- ing 0.5 micromolar boric acid, 1.5% gellan gum and 1% sucrose for 4 or 5 days.
Organism
Arabidopsis thaliana (NCBITaxon:3702)
Strain
Col-0 (https://arapheno.1001genomes.org/accession/6909/)
Cell
epidermal cell (CL:0000362)
Cell line
-
MeSH
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Plant Roots (D018517)
Protein Transport (D021381)
Intracellular Membranes (D007425)
Biological Transport (D001692)
Time-Lapse Imaging (D059008)
Cell Membrane (D002462)
Vacuoles (D014617)
Intrinsic Variable
A. thaliana which was mutated in AP-4 complex subunit beta
Extrinsic Variable
BOR1-GFP was exogenously expressed. A. thaliana roots were treated with 100 micromolar boric acid at the start of imaging.

Ontology

Anatomical Entity
root (PO:0009005)
root epidermis (PO:0006036)
UBERON
-
Biological Process
vesicle-mediated transport (GO:0016192)
intracellular borate homeostasis (GO:0160070)
Cellular Component
vesicle membrane (GO:0012506)
plasma membrane (GO:0005886)
vacuole (GO:0005773)
Molecular Function
active borate transmembrane transporter activity (GO:0046715)
MeSH Anatomy
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Plant Roots (D018517)
Protein Transport (D021381)
Intracellular Membranes (D007425)
Biological Transport (D001692)
Time-Lapse Imaging (D059008)
Cell Membrane (D002462)
Vacuoles (D014617)
MeSH BP
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Plant Roots (D018517)
Protein Transport (D021381)
Intracellular Membranes (D007425)
Biological Transport (D001692)
Time-Lapse Imaging (D059008)
Cell Membrane (D002462)
Vacuoles (D014617)
MeSH CC
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Plant Roots (D018517)
Protein Transport (D021381)
Intracellular Membranes (D007425)
Biological Transport (D001692)
Time-Lapse Imaging (D059008)
Cell Membrane (D002462)
Vacuoles (D014617)
MeSH MF
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Plant Roots (D018517)
Protein Transport (D021381)
Intracellular Membranes (D007425)
Biological Transport (D001692)
Time-Lapse Imaging (D059008)
Cell Membrane (D002462)
Vacuoles (D014617)

Genetics

Gene
-
Protein / Probe
Boron transporter 1
Method / Genotype
binary vector method
Gene
-
Protein / Probe
-FM4-64
Method / Genotype
-
Gene
AT5G11490
Protein / Probe
-
Method / Genotype
-ap4b-2

Treatments

Reagent / Compound
boric acid
Concentration
100 micromolar
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy (FBbi:00000251)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
Green fluorescent protein from Aequorea FM4-64; a probe for cell membrane
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Plant Roots (D018517)
Protein Transport (D021381)
Intracellular Membranes (D007425)
Biological Transport (D001692)
Time-Lapse Imaging (D059008)
Cell Membrane (D002462)
Vacuoles (D014617)

Instrument

Body
Zeiss LSM800
Module
-
Light Source
-
Detector
-
Objective
Zeiss LD C-Apochromat 40x/1.1 W
Filter Set
-
Dichroic
-