402-Tasaka-parentOFCactivity/Fig2_Rbp4_Ai162_MD_F6_210330_AP2
Author reviewedTime-lapse calcium imaging showing the neural activity in OFC of female mouse at alloparental day 2
Project
Dataset ID
Fig2_Rbp4_Ai162_MD_F6_210330_AP2
Kind
Image data
Title
Time-lapse calcium imaging showing the neural activity in OFC of female mouse at alloparental day 2
Description
The authors investigated the neural activity of the orbitofrontal cortex (OFC) during the parental behavior of retrieving pups at three maternal stages: AP1 (alloparental day 1), AP2 (alloparental day 1), and LM (lactating mother stage). A virgin female showing more than five pup-retrievals per session for the first time was defined as AP1 and the day following AP1 was defined as AP2. At AP1 and AP2, the pup-retrievals were examined using pups born from another female mouse. For the calcium imaging, the transgenic female mouse (Rbp4-Cre; Ai162 mouse) expressing GCaMP6s in the layer 5 pyramidal cells of the orbitofrontal cortex was used. The signal of GCaMP6s was obtained through gradient refractive index (GRIN) lens (500 micrometres in diameter) implanted above layer 5 of the OFC. This dataset includes the imaging data at AP2. The data at AP1 and LM obtained from the same female mouse are deposited in other datasets in this project; Fig2_Rbp4_Ai162_MD_F6_210329_AP1, and Fig2_Rbp4_Ai162_MD_F6_210421_LM.
Folder Name
Fig2_Rbp4_Ai162_MD_F6_210330_AP2
File Format
tiff
File Size
22.8 GB
File Separation Method
-
Figure Number
Figure2
Dimensions
800x700x1x1x21602
X Scale
0.625 micrometer
Y Scale
0.602 micrometer
Z Scale
-
T Scale
0.1 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)
Biosample
Description
The transgenic female mouse, Rbp4-Cre; Ai162 female mouse, was surgically implanted with a gradient refractive index (GRIN) lens (500 micrometres in diameter) above layer 5 of the OFC to monitor the OFCRbp4 neuron activity.
Organism
Mus musculus
Strain
-
Cell
pyramidal neuron
cerebral cortex pyramidal neuron
L5 extratelencephalic projecting glutamatergic cortical neuron (CL:4023041)
Cell line
-
Intrinsic Variable
Rbp4-Cre mice were crossed with the Ai162 reporter line, which expresses GCaMP6s in a Cre-dependent manner.
GCaMP6s is expressed specifically in the layer 5 pyramidal neurons of the orbitofrontal cortex.
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
orbitofrontal cortex (UBERON:0004167)
brain (UBERON:0000955)
Biological Process
-
Cellular Component
-
Molecular Function
-
Genetics
Gene
-
Protein / Probe
-GCaMP6s
Method / Genotype
transgenic / Ai162(TIT2L-GC6s-ICL-tTA2)-D (or Ai162D)
B6.FVB(Cg)-Tg(Rbp4-cre)KL100Gsat/Mmucd
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
Detection Method
complementary metal oxide semiconductor (CMOS)
Visualization Method
GCaMP6s
Illumination Method
-
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
Implantation of imaging devices (GRIN lens) in mouse brain
Instrument
Body
-
Module
-
Light Source
LED
Detector
Inscopix nVista system
Objective
ProView GRIN lens (500 μm in diameter, 4 mm in length, Inscopix)
Filter Set
-
Dichroic
-