402-Tasaka-parentOFCactivity/Fig2_Rbp4_Ai162_Gi_SMTb_F4_211112_LM
Author reviewedTime-lapse calcium imaging showing the neural activity in OFC of female mouse at LM
Project
Dataset ID
Fig2_Rbp4_Ai162_Gi_SMTb_F4_211112_LM
Kind
Image data
Title
Time-lapse calcium imaging showing the neural activity in OFC of female mouse at LM
Description
The authors investigated the neural activity of the orbitofrontal cortex (OFC) during the parental behavior of retrieving pups at three maternal stages: AP1 (alloparental day 1), AP2 (alloparental day 1), and LM (lactating mother stage). A virgin female showing more than five pup-retrievals per session for the first time was defined as AP1 and the day following AP1 was defined as AP2. The pup-retrievals were examined using her own pups at LM. For the calcium imaging, the transgenic female mouse (Rbp4-Cre; Ai162 mouse) expressing GCaMP6s in the layer 5 pyramidal cells of the orbitofrontal cortex was used. The signal of GCaMP6s was obtained through gradient refractive index (GRIN) lens (500 micrometres in diameter) implanted above layer 5 of the OFC. This dataset includes the imaging data at LM. The data at AP1 and AP2 obtained from the same female mouse are deposited in other datasets in this project; Fig2_Rbp4_Ai162_Gi_SMTb_F4_211012_AP1, and Fig2_Rbp4_Ai162_Gi_SMTb_F4_211013_AP2.
Folder Name
Fig2_Rbp4_Ai162_Gi_SMTb_F4_211112_LM
File Format
tiff
File Size
15.2 GB
File Separation Method
-
Figure Number
Figure2
Dimensions
800x700x1x1x14401
X Scale
0.625 micrometer
Y Scale
0.602 micrometer
Z Scale
-
T Scale
0.1 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)
Biosample
Description
The transgenic female mouse, Rbp4-Cre; Ai162 female mouse, was surgically implanted with a gradient refractive index (GRIN) lens (500 micrometres in diameter) above layer 5 of the OFC to monitor the OFCRbp4 neuron activity.
Organism
Mus musculus
Strain
-
Cell
pyramidal neuron
cerebral cortex pyramidal neuron
L5 extratelencephalic projecting glutamatergic cortical neuron (CL:4023041)
Cell line
-
Intrinsic Variable
Rbp4-Cre mice were crossed with the Ai162 reporter line, which expresses GCaMP6s in a Cre-dependent manner.
GCaMP6s is expressed specifically in the layer 5 pyramidal neurons of the orbitofrontal cortex.
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
orbitofrontal cortex (UBERON:0004167)
brain (UBERON:0000955)
Biological Process
-
Cellular Component
-
Molecular Function
-
Genetics
Gene
-
Protein / Probe
-GCaMP6s
Method / Genotype
transgenic / Ai162(TIT2L-GC6s-ICL-tTA2)-D (or Ai162D)
B6.FVB(Cg)-Tg(Rbp4-cre)KL100Gsat/Mmucd
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
Detection Method
complementary metal oxide semiconductor (CMOS)
Visualization Method
GCaMP6s
Illumination Method
-
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
Implantation of imaging devices (GRIN lens) in mouse brain
Instrument
Body
-
Module
-
Light Source
LED
Detector
Inscopix nVista system
Objective
ProView GRIN lens (500 μm in diameter, 4 mm in length, Inscopix)
Filter Set
-
Dichroic
-