386-Takahashi-EarIL31RA/Fig1B_IL31RA_ctrl_1
Author reviewedZ-series immunofluorescence images of the ear skin from control mouse
Project
Dataset ID
Fig1B_IL31RA_ctrl_1
Kind
Image data
Title
Z-series immunofluorescence images of the ear skin from control mouse
Description
Z-series immunofluorescence images of the ear skin from control mouse. The skin was stained with anti-IL31 RA (Interleukin 31 Receptor A), and DAPI. tdTomato-labeled sensory neuron fibers heavily innervate the epidermis, wheres there are almost no IL-31 RA-positive nerves found in the epidermis.
Chennel1;DAPI, Channel2;IL31-RA, Channel3;tdTomato,
Channel4;betaIII-tubulin
Folder Name
Fig1B_IL31RA_ctrl_1
File Format
.lif
File Size
94MB
File Separation Method
-
Figure Number
Fig1B
Dimensions
512x512x94x4x1
X Scale
0.18 micrometer
Y Scale
0.18 micrometer
Z Scale
0.5 micrometer
T Scale
-
Channel
4 channel
Quantitative Data
-
Paper(s)
Biosample
Description
Mouse ear pinnae were harvested and split into dorsal and ventral layers. The ventral layer with the cartilage was used to whole mount immunofluorescence staining of ear skin tissue.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron,
keratinocyte (CL:0000312)
Cell line
-
MeSH
Intrinsic Variable
C- and A-delta-fiber sensory neurons were labeled with tdTomato
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
skin of external ear (UBERON:0001459)
Biological Process
-
Cellular Component
axon (GO:0030424)
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-DAPI
Method / Genotype
-B6.129-Scn10a^{tm2(cre)Jwo}/H,
B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J
Gene
-
Protein / Probe
dTomatoFP / antibody
Method / Genotype
-B6.129-Scn10a^{tm2(cre)Jwo}/H,
B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J
Gene
-
Protein / Probe
Interleukin-31 receptor subunit alpha / antibody
Method / Genotype
-B6.129-Scn10a^{tm2(cre)Jwo}/H,
B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J
Gene
-
Protein / Probe
Tubulin beta-3 chain / antibody
Method / Genotype
-B6.129-Scn10a^{tm2(cre)Jwo}/H,
B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
photomultiplier tube (PMT)
Visualization Method
DAPI,
Alexa Flour 488,
Alexa Flour 555,
Alexa Flour 647
Illumination Method
single point scanning
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
4% PFA fixed whole mounted tissue,
MeSH
Instrument
Body
Leica TCS SP8
Module
-
Light Source
405-nm diode laser, 488-nm OPSL, a 552-nm OPSL, 638-nm diode laser
Detector
Leica HyD
Objective
HC PL APO CS2 40x/1.30 OIL lens
Filter Set
414–448nm emission filter for DAPI; 498-538nm emission filter for Alexa Flour 488; 555-597nm emission filter for Alexa Flour 555; 653–800nm emission filter for Alexa Flour 647
Dichroic
-