SSBD:database

367-Tsuboi-DrosophilaWing/MovieS9_DpyOE

Author reviewed

Z-series and time-lapse images of wings expressing mCD8::mCherry and Dpy::YFP in Dpy overexpressing Drosophila pupa

SSBD:downloads

Project

367-Tsuboi-DrosophilaWing

Dataset ID

MovieS9_DpyOE

Kind

Image data

Title

Z-series and time-lapse images of wings expressing mCD8::mCherry and Dpy::YFP in Dpy overexpressing Drosophila pupa

Description

Z-series and time-lapse images of wings expressing mCD8::mCherry induced by hh-Gal4 and endogenous Dpy::YFP in Dumpy (Dpy) overexpressing pupa at the posterior region of the wing. Channel1; YFP, Channel2; mCherry

Folder Name

MovieS9_DpyOE

File Format

.tif

File Size

18.4GB

File Separation Method

Figure Number

MovieS9, FigS8

Dimensions

1024x1024x100x2x47

X Scale

0.624 micrometer

Y Scale

0.624 micrometer

Z Scale

2 micrometer

T Scale

20 minutes

Channel

2 channel

Quantitative Data

Paper(s)

Biosample

Description

A Drosophila pupa expressing mCD8::mCherry and Dpy::YFP in its wing. In addition, Dumpy (Dpy) was overexpressed at the posterior region of its wing.

Organism

Drosophila (NCBITaxon:7215)

Strain

Cell

Cell line

MeSH

Microscopy, Confocal (D018613)

Pupa (D011679)

growth & development (Q000254)

Extracellular Matrix (D005109)

Intrinsic Variable

A transgenic pupa expressing mCD8::mCherry and Dpy::YFP in its wing. In addition, Dpy was overexpressed at the posterior region of its wing.

Extrinsic Variable

Ontology

Anatomical Entity

pupa (FBbt:00002953)

wing (FBbt:00004729)

UBERON

pupa (UBERON:0003143)

wing (UBERON:0000023)

Biological Process

pupal development (GO:0035209)

pattern specification process (GO:0007389)

Cellular Component

extracellular matrix (GO:0031012)

Molecular Function

MeSH Anatomy

Microscopy, Confocal (D018613)

Pupa (D011679)

growth & development (Q000254)

Extracellular Matrix (D005109)

MeSH BP

Microscopy, Confocal (D018613)

Pupa (D011679)

growth & development (Q000254)

Extracellular Matrix (D005109)

MeSH CC

Microscopy, Confocal (D018613)

Pupa (D011679)

growth & development (Q000254)

Extracellular Matrix (D005109)

MeSH MF

Microscopy, Confocal (D018613)

Pupa (D011679)

growth & development (Q000254)

Extracellular Matrix (D005109)

Genetics

Gene

Protein / Probe

SP460

Method / Genotype

transgenic / Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; hh-Gal4/ UAS-3xFLAG.dCas9.VPR

Gene

Protein / Probe

T-cell surface glycoprotein CD8 alpha chain

Method / Genotype

transgenic / Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; hh-Gal4/ UAS-3xFLAG.dCas9.VPR

Gene

dpy

Protein / Probe

Method / Genotype

transgenic / Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; hh-Gal4/ UAS-3xFLAG.dCas9.VPR

Treatments

Reagent / Compound

Concentration

Fold Dilution

Imaging Method

confocal microscopy (FBbi:00000251)

time lapse microscopy (FBbi:00000249)

Detection Method

Visualization Method

YFP, mCherry

Illumination Method

Source of Contrast

distribution of a specific protein

Imaged Parameter

fluorescence emission

Sample Preparation Method

living tissue

MeSH

Microscopy, Confocal (D018613)

Pupa (D011679)

growth & development (Q000254)

Extracellular Matrix (D005109)

Instrument

Body

Zeiss LSM 800

Module

Light Source

Detector

Objective

Filter Set

Dichroic