SSBD:database

367-Tsuboi-DrosophilaWing/MovieS7_DpyKD_dorsal

Author reviewed
Z-series and time-lapse images of Dpy-depleted Drosophila wing
SSBD:downloads
Project
367-Tsuboi-DrosophilaWing
Dataset ID
MovieS7_DpyKD_dorsal
Kind
Image data
Title
Z-series and time-lapse images of Dpy-depleted Drosophila wing
Description
Z-series and time-lapse images of Drosophila wing expressing DsRed in dorsal cells of its wing. The Drosophila pupa was depleted Dumpy (Dpy) in the dorsal wing.
Folder Name
MovieS7_DpyKD_dorsal
File Format
.tif
File Size
3.7GB
File Separation Method
-
Figure Number
MovieS7, Fig2DE
Dimensions
1024x1024x55x1x34
X Scale
0.692 micrometer
Y Scale
0.692 micrometer
Z Scale
３ micrometer
T Scale
20 minutes
Channel
1 channel
Quantitative Data
Yes
Paper(s)

Biosample

Description
A Drosophila pupa that DsRed was expressed in dorsal cells of its wing and Dumpy was depleted from the dorsal wing.
Organism
Drosophila (NCBITaxon:7215)
Strain
-
Cell
-
Cell line
-
MeSH
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)
Intrinsic Variable
A transgenic pupa that DsRed was expressed in its wing and Dpy was depleted from the dorsal wing.
Extrinsic Variable
-

Ontology

Anatomical Entity
pupa (FBbt:00002953)
wing (FBbt:00004729)
UBERON
pupa (UBERON:0003143)
wing (UBERON:0000023)
Biological Process
pupal development (GO:0035209)
pattern specification process (GO:0007389)
Cellular Component
extracellular matrix (GO:0031012)
Molecular Function
-
MeSH Anatomy
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)
MeSH BP
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)
MeSH CC
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)
MeSH MF
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / ap-GAL4/UAS-dpyRNAi; UAS-Dcr2/UAS-DsRed
Gene
dpy
Protein / Probe
-
Method / Genotype
transgenic / ap-GAL4/UAS-dpyRNAi; UAS-Dcr2/UAS-DsRed

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy (FBbi:00000251)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
DsRed
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)

Instrument

Body
Zeiss LSM 710
Module
-
Light Source
-
Detector
-
Objective
-
Filter Set
-
Dichroic
-