SSBD:database

367-Tsuboi-DrosophilaWing/MovieS5_Dpy_2

Author reviewed
Z-series images of Drosophila prefolded wing expressing mCD8::mCherry and Dpy::YFP
SSBD:downloads
Project
367-Tsuboi-DrosophilaWing
Dataset ID
MovieS5_Dpy_2
Kind
Image data
Title
Z-series images of Drosophila prefolded wing expressing mCD8::mCherry and Dpy::YFP
Description
Z-stack images of a control prefolded wing expressing mCD8::mCherry induced by sd-Gal4 and endogenous Dpy::YFP. Dpy is Dumpy. Channel1; YFP, Channel2; mCherry
Folder Name
MovieS5_Dpy_2
File Format
.oif
File Size
100MB
File Separation Method
-
Figure Number
MovieS5, Fig2A
Dimensions
512x512x100x2x1
X Scale
0.83 micrometer
Y Scale
0.83 micrometer
Z Scale
2 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
A wing of Drosophila pupa expressing mCD8::mCherry and Dpy::YFP.
Organism
Drosophila (NCBITaxon:7215)
Strain
-
Cell
-
Cell line
-
MeSH
Microscopy, Fluorescence, Multiphoton (D036641)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)
Intrinsic Variable
A wing of transgenic pupa expressing mCD8::mCherry and Dpy::YFP.
Extrinsic Variable
-

Ontology

Anatomical Entity
pupa (FBbt:00002953)
wing (FBbt:00004729)
UBERON
pupa (UBERON:0003143)
wing (UBERON:0000023)
Biological Process
pupal development (GO:0035209)
pattern specification process (GO:0007389)
Cellular Component
extracellular matrix (GO:0031012)
Molecular Function
-
MeSH Anatomy
Microscopy, Fluorescence, Multiphoton (D036641)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)
MeSH BP
Microscopy, Fluorescence, Multiphoton (D036641)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)
MeSH CC
Microscopy, Fluorescence, Multiphoton (D036641)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)
MeSH MF
Microscopy, Fluorescence, Multiphoton (D036641)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)

Genetics

Gene
-
Protein / Probe
SP460
Method / Genotype
transgenic / sd-Gal4/+; Dpy::YFP, UAS-mCD8::mCherry/Dpy::YFP
Gene
-
Protein / Probe
T-cell surface glycoprotein CD8 alpha chain
Method / Genotype
transgenic / sd-Gal4/+; Dpy::YFP, UAS-mCD8::mCherry/Dpy::YFP

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
multi-photon microscopy (FBbi:00000255)
Detection Method
-
Visualization Method
YFP, mCherry
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Microscopy, Fluorescence, Multiphoton (D036641)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)

Instrument

Body
Olympus FV1000MPE-IX83 MP mi- croscope
Module
-
Light Source
-
Detector
-
Objective
-
Filter Set
-
Dichroic
-