367-Tsuboi-DrosophilaWing/MovieS4_FoldingWing_sqhKO_Post
Author reviewed
Z-series and time-lapse images of a folding wing in sqh-KO Drosophila pupa
Dataset ID
MovieS4_FoldingWing_sqhKO_Post
Title
Z-series and time-lapse images of a folding wing in sqh-KO Drosophila pupa
Description
Z-series and time-lapse imaging of Drosophila wing in NSlmb-vhhGFP4-mediated knockdown of Sqh::eGFP at posterior region of the wing expressing mCD8::mCherry induced by hh-Gal4. Sqh (spaghetti squash) encodes myosin II regulatory light chain.
Channel1; GFP, Channel2; mCherry
Folder Name
MovieS4_FoldingWing_sqhKO_Post
Figure Number
MovieS4, FigS6
Dimensions
512x512x100x2x54
Biosample
Description
A Drosophila pupa that sqh was knocked out at posterior region of the wing.
Intrinsic Variable
A transgenic pupa that sqh was knocked out at posterior region of the wing.
Ontology
Genetics
Protein / Probe
Myosin regulatory light chain sqh
Method / Genotype
transgenic / y, w, sqh::eGFP/Y; Tub-Gal80ts/+; UAS-Nslmb-vhhGFP4, UAS- mCD8::mCherry/hh-Gal4
Protein / Probe
T-cell surface glycoprotein CD8 alpha chain
Method / Genotype
transgenic / y, w, sqh::eGFP/Y; Tub-Gal80ts/+; UAS-Nslmb-vhhGFP4, UAS- mCD8::mCherry/hh-Gal4
Treatments
Imaging Method
Visualization Method
GFP,
mCherry
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
Instrument