367-Tsuboi-DrosophilaWing/MovieS2_CellExpand

Author reviewed
Z-series and time-lapse images of a folding wing in Drosophila pupa expressing E-Cadherin-GFP
Project
Dataset ID
MovieS2_CellExpand
Kind
Image data
Title
Z-series and time-lapse images of a folding wing in Drosophila pupa expressing E-Cadherin-GFP
Description
Z-series and time-lapse images of a folding wing in Drosophila pupa expressing mCD8::mCherry and E-Cadherin-GFP. Channel1; GFP, Channel2; mCherry
Folder Name
MovieS2_CellExpand
File Format
.czi
File Size
17.2GB, 17.2GB
File Separation Method
series1 and series2
Figure Number
MovieS2, FigS3
Dimensions
1024x1024x100x2x44, 1024x1024x100x2x44
X Scale
0.312 micrometer, 0.312 micrometer
Y Scale
0.312 micrometer, 0.312 micrometer
Z Scale
1 micrometer
T Scale
20 minutes
Channel
2 channel
Quantitative Data
Yes

Paper(s)

Biosample

Description
A Drosophila pupa expressing mCD8::mCherry and E-Cadherin-GFP in its wing.
Organism
Drosophila (NCBITaxon:7215)
Strain
-
Cell
-
Cell line
-
MeSH
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Cell Membrane (D002462)
Intrinsic Variable
A transgenic pupa expressing mCD8::mCherry and E-Cadherin-GFP in cells of wing.
Extrinsic Variable
-

Ontology

Anatomical Entity
UBERON
Biological Process
pupal development (GO:0035209)
pattern specification process (GO:0007389)
Cellular Component
plasma membrane (GO:0005886)
Molecular Function
-
MeSH Anatomy
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Cell Membrane (D002462)
MeSH BP
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Cell Membrane (D002462)
MeSH CC
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Cell Membrane (D002462)
MeSH MF
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Cell Membrane (D002462)

Genetics

Gene
-
Protein / Probe
DE-cadherin
Method / Genotype
transgenic / y, w, hs-flp; E-Cadherin::GFP; Act > CD2 > GAL4; UAS-mCD8::mCherry

Gene
-
Protein / Probe
T-cell surface glycoprotein CD8 alpha chain
Method / Genotype
transgenic / y, w, hs-flp; E-Cadherin::GFP; Act > CD2 > GAL4; UAS-mCD8::mCherry

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy (FBbi:00000251)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
GFP, mCherry
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Cell Membrane (D002462)

Instrument

Body
Zeiss LSM 800
Module
-
Light Source
-
Detector
-
Objective
-
Filter Set
-
Dichroic
-