367-Tsuboi-DrosophilaWing/MovieS10_ctrl

Author reviewed
Z-series and time-lapse images of wings expressing Gap43::tdTomato and Dpy::YFP in control Drosophila pupa
Project
Dataset ID
MovieS10_ctrl
Kind
Image data
Title
Z-series and time-lapse images of wings expressing Gap43::tdTomato and Dpy::YFP in control Drosophila pupa
Description
Z-series and time-lapse images of wings expressing Gap43::tdTomato and Dpy::YFP in control Drosophila pupa. Channel1; YFP, Channel2; tdTomato
Folder Name
MovieS10_ctrl
File Format
.tif
File Size
19.1GB
File Separation Method
-
Figure Number
MovieS10, FigS9
Dimensions
1024x1024x100x2x49
X Scale
0.624 micrometer
Y Scale
0.624 micrometer
Z Scale
2 micrometer
T Scale
20 minutes
Channel
2 channel
Quantitative Data
-

Paper(s)

Biosample

Description
A control Drosophila pupa expressing Gap43::tdTomato and Dpy::YFP in the wing. tdTomato was fused with N-terminal region of GAP43.
Organism
Drosophila (NCBITaxon:7215)
Strain
-
Cell
-
Cell line
-
MeSH
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)
Intrinsic Variable
A control Drosophila pupa expressing Gap43::tdTomato and Dpy::YFP in the wing
Extrinsic Variable
-

Ontology

Anatomical Entity
UBERON
Biological Process
pupal development (GO:0035209)
pattern specification process (GO:0007389)
Cellular Component
extracellular matrix (GO:0031012)
Molecular Function
-
MeSH Anatomy
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)
MeSH BP
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)
MeSH CC
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)
MeSH MF
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)

Genetics

Gene
-
Protein / Probe
SP460
Method / Genotype
transgenic / Dpy::YFP, Ubi-GAP43:: tdTomato/+; rho^{ve-1}, vn^{1}/+

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / Dpy::YFP, Ubi-GAP43:: tdTomato/+; rho^{ve-1}, vn^{1}/+

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy (FBbi:00000251)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
YFP, tdTomato
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Microscopy, Confocal (D018613)
Pupa (D011679)
growth & development (Q000254)
Extracellular Matrix (D005109)

Instrument

Body
Zeiss LSM 800
Module
-
Light Source
-
Detector
-
Objective
-
Filter Set
-
Dichroic
-