SSBD:database

367-Tsuboi-DrosophilaWing/FigS19_NpKD

Author reviewed

Z-series and time-lapse images of wings in Np knockdown pupa expressing mCD8::mCherry and E-Cadherin::GFP

SSBD:downloads

Project

367-Tsuboi-DrosophilaWing

Dataset ID

FigS19_NpKD

Kind

Image data

Title

Z-series and time-lapse images of wings in Np knockdown pupa expressing mCD8::mCherry and E-Cadherin::GFP

Description

Z-series and time-lapse images of wings in Notopleural (Np) knockdown Drosophila pupa expressing mCD8::mCherry induced by sd-Gal4 and E-Cadherin::GFP during wing folding. Channel1; GFP, Channel2; mCherry

Folder Name

FigS19_NpKD

File Format

.czi

File Size

10GB, 11.1GB

File Separation Method

series1 and series2

Figure Number

FigS19

Dimensions

1024x1024x150x2x17, 1024x1024x150x2x19

X Scale

0.312 micrometer

Y Scale

0.312 micrometer

Z Scale

1 micrometer

T Scale

1 hour

Channel

2 channel

Quantitative Data

Yes

Paper(s)

Biosample

Description

A Drosophila pupa expressing mCD8::mCherry and E-Cadherin::GFP. Notopleural was depleted from the wing.

Organism

Drosophila (NCBITaxon:7215)

Strain

Cell

Cell line

MeSH

Microscopy, Confocal (D018613)

Pupa (D011679)

growth & development (Q000254)

Cell Membrane (D002462)

Intrinsic Variable

A trsnsgenic Drosophila pupa expressing mCD8::mCherry and E-Cadherin::GFP. Notopleural was depleted from the wing.

Extrinsic Variable

Ontology

Anatomical Entity

pupa (FBbt:00002953)

wing (FBbt:00004729)

UBERON

pupa (UBERON:0003143)

wing (UBERON:0000023)

Biological Process

pupal development (GO:0035209)

pattern specification process (GO:0007389)

Cellular Component

plasma membrane (GO:0005886)

Molecular Function

MeSH Anatomy

Microscopy, Confocal (D018613)

Pupa (D011679)

growth & development (Q000254)

Cell Membrane (D002462)

MeSH BP

Microscopy, Confocal (D018613)

Pupa (D011679)

growth & development (Q000254)

Cell Membrane (D002462)

MeSH CC

Microscopy, Confocal (D018613)

Pupa (D011679)

growth & development (Q000254)

Cell Membrane (D002462)

MeSH MF

Microscopy, Confocal (D018613)

Pupa (D011679)

growth & development (Q000254)

Cell Membrane (D002462)

Genetics

Gene

Protein / Probe

DE-cadherin

Method / Genotype

transgenic / sd-Gal4/+; UAS-mCD8::mCherry/E- Cadherin::GFP; UAS-NpRNAi/+

Gene

Protein / Probe

T-cell surface glycoprotein CD8 alpha chain

Method / Genotype

transgenic / sd-Gal4/+; UAS-mCD8::mCherry/E- Cadherin::GFP; UAS-NpRNAi/+

Gene

Protein / Probe

Method / Genotype

transgenic / sd-Gal4/+; UAS-mCD8::mCherry/E- Cadherin::GFP; UAS-NpRNAi/+

Treatments

Reagent / Compound

Concentration

Fold Dilution

Imaging Method

confocal microscopy (FBbi:00000251)

time lapse microscopy (FBbi:00000249)

Detection Method

Visualization Method

mCherry, GFP

Illumination Method

Source of Contrast

distribution of a specific protein

Imaged Parameter

fluorescence emission

Sample Preparation Method

living tissue

MeSH

Microscopy, Confocal (D018613)

Pupa (D011679)

growth & development (Q000254)

Cell Membrane (D002462)

Instrument

Body

Zeiss LSM 800

Module

Light Source

Detector

Objective

Filter Set

Dichroic