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289-Fujimoto-MitralCell/FigS6I_Grin1Pcdh_mut_2 thumbnail

289-Fujimoto-MitralCell/FigS6I_Grin1Pcdh_mut_2

Author reviewed
Z-series images of mitral cells labed by tdTomato in which Grin1 was knocked out specifically
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Project
289-Fujimoto-MitralCell
Dataset ID
FigS6I_Grin1Pcdh_mut_2
Kind
Image data
Title
Z-series images of mitral cells labed by tdTomato in which Grin1 was knocked out specifically
Description
Z-series images of mitral cells labed by tdTomato in which Grin1 was knocked out specifically. Grin1 codes NR1, an essential subunit of NMDA receptors.
Folder Name
FigS6I_Grin1Pcdh_mut_2
File Format
.oif
File Size
606MB
File Separation Method
-
Figure Number
FigS6I
Dimensions
3070x1024x101x1x1
X Scale
0.497 micrometer
Y Scale
0.497 micrometer
Z Scale
10 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
A fixed and cleared olfactory bulb of P6 mouse in which Grin1 was knocked out specifically in mitral/tufted cells. tdTomato was expressed in mitral/tufted cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
Intrinsic Variable
floxed-Grin1 mice with expression of Cre. By expressing Cre in M/T cells, Grin1 was knocked out specifically in M/T cells
Extrinsic Variable
By using in utero electroporation, the authors electroporated FLP (pCAG-Flpo) and FLP-dependent tdTomato plasmid (pCAFNF-tdTomato) at E12.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development neuron remodeling (GO:0016322)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation / B6.129S4-Grin1^{tm2Stl}/J C57BL/6Cr-Tg(Pcdh21-cre)BYoko
Gene
Grin1
Protein / Probe
-
Method / Genotype
transgenic / B6.129S4-Grin1^{tm2Stl}/J C57BL/6Cr-Tg(Pcdh21-cre)BYoko

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
tdTomatoFP
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue vibratome-sectioned tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Instrument

Body
Olympus, FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XSVMP, NA 1.0, WD 4.0 mm
Filter Set
-
Dichroic
-