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289-Fujimoto-MitralCell/FigS6I_Grin1Pcdh_ctrl_3 thumbnail

289-Fujimoto-MitralCell/FigS6I_Grin1Pcdh_ctrl_3

Author reviewed
Z-series images of P6 mouse brain in which mitral cells were labed by tdTomato
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Project
289-Fujimoto-MitralCell
Dataset ID
FigS6I_Grin1Pcdh_ctrl_3
Kind
Image data
Title
Z-series images of P6 mouse brain in which mitral cells were labed by tdTomato
Description
Z-series images of P6 mouse brain in which mitral cells were labed by tdTomato. The control experiment for mitral/tufted cell-specific Grin1 knockout.
Folder Name
FigS6I_Grin1Pcdh_ctrl_3
File Format
.oif
File Size
606MB
File Separation Method
-
Figure Number
FigS6I
Dimensions
3070x1024x101x1x1
X Scale
0.497 micrometer
Y Scale
0.497 micrometer
Z Scale
10 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
A fixed and cleared olfactory bulb of P6 mouse. tdTomato was expressed in mitral/tufted cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
Intrinsic Variable
floxed-Grin1 mice without expression of Cre
Extrinsic Variable
By using in utero electroporation, the authors electroporated FLP (pCAG-Flpo) and FLP-dependent tdTomato plasmid (pCAFNF-tdTomato) and at E12.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development neuron remodeling (GO:0016322)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation / B6.129S4-Grin1^{tm2Stl}/J

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
tdTomatoFP
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue vibratome-sectioned tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Instrument

Body
Olympus, FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XSVMP, NA 1.0, WD 4.0 mm
Filter Set
-
Dichroic
-