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289-Fujimoto-MitralCell/FigS6H_Grin1_lowCre_1 thumbnail

289-Fujimoto-MitralCell/FigS6H_Grin1_lowCre_1

Author reviewed
Z-series images of Grin1 conditional knockout mouse brain at P6 in which mitral cells were labed by tdTomato
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Project
289-Fujimoto-MitralCell
Dataset ID
FigS6H_Grin1_lowCre_1
Kind
Image data
Title
Z-series images of Grin1 conditional knockout mouse brain at P6 in which mitral cells were labed by tdTomato
Description
Z-series images of Grin1 conditional knockout mouse brain at P6 in which mitral cells were labed by tdTomato. Grin1 codes NR1, an essential subunit of NMDA receptors.
Folder Name
FigS6H_Grin1_lowCre_1
File Format
.oif
File Size
826MB
File Separation Method
-
Figure Number
FigS6H
Dimensions
3070x1024x131x1x1
X Scale
0.497 micrometer
Y Scale
0.497 micrometer
Z Scale
10 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
A fixed and cleared olfactory bulb of P6 mouse in which Grin1 was knocked out sparsely. tdTomato was expressed in mitral/tufted cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
Intrinsic Variable
floxed-Grin1 mice, in which Grin1 was knocked out sparsely in mitral/tufted cells by expressing pCAG-Cre.
Extrinsic Variable
By using in utero electroporation, the authors electroporated a tdTomato plasmid (pCALNL-tdTomato) and Cre plasmid (pCAG-Cre) at E12.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development neuron remodeling (GO:0016322)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation / B6.129S4-Grin1^{tm2Stl}/J
Gene
Grin1
Protein / Probe
-
Method / Genotype
transgenic / B6.129S4-Grin1^{tm2Stl}/J

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
tdTomatoFP
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue vibratome-sectioned tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Instrument

Body
Olympus, FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XSVMP, NA 1.0, WD 4.0 mm
Filter Set
-
Dichroic
-