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289-Fujimoto-MitralCell/FigS6CD_TeNT_20121116_pup2_1 thumbnail

289-Fujimoto-MitralCell/FigS6CD_TeNT_20121116_pup2_1

Author reviewed
Time-lapse calcium imaging of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells with expression of M/T-TeNT in the cells
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Project
289-Fujimoto-MitralCell
Dataset ID
FigS6CD_TeNT_20121116_pup2_1
Kind
Image data
Title
Time-lapse calcium imaging of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells with expression of M/T-TeNT in the cells
Description
Time-lapse calcium imaging of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells (M/T cells), and TeNT (tetanus toxin light chain) was expressed in the M/T cells.
Folder Name
FigS6CD_TeNT_20121116_pup2_1
File Format
.oib
File Size
175MB
File Separation Method
-
Figure Number
FigS6CD
Dimensions
256x256x1x1x1398
X Scale
1.99 micrometer
Y Scale
1.99 micrometer
Z Scale
-
T Scale
0.429 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Live and anesthetized P6 mouse expressing GCaMP6f and tetanus toxin light chain in mitral/tufted cells
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
Intrinsic Variable
A mouse line Thy1-GCaMP6f (C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J) in which GCaMP6f is expressed in neurons. Pcdh21-Cre;R26-CAG-loxP-TeNT (designated M/T-TeNT) mice, in which TeNT is specifically expressed in mitral/tufted cells.
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J C57BL/6Cr-Tg(Pcdh21-cre)BYoko B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-EGFP/TeNT)Imayo>
Gene
-
Protein / Probe
Tetanus toxin
Method / Genotype
transgenic / C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J C57BL/6Cr-Tg(Pcdh21-cre)BYoko B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-EGFP/TeNT)Imayo>

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-