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289-Fujimoto-MitralCell/FigS5D_AP5_141104 thumbnail

289-Fujimoto-MitralCell/FigS5D_AP5_141104

Author reviewed
Time-lapse calcium imaging of olfactory bulb slice with AP5 treatment from P2-3 mouse expressing GCaMP6f in mitral/tufted cells
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Project
289-Fujimoto-MitralCell
Dataset ID
FigS5D_AP5_141104
Kind
Image data
Title
Time-lapse calcium imaging of olfactory bulb slice with AP5 treatment from P2-3 mouse expressing GCaMP6f in mitral/tufted cells
Description
Time-lapse calcium imaging of olfactory bulb slice from P2-3 mouse expressing GCaMP6f in mitral/tufted cells. The brain slice was treated with 50 micromolar AP5.
Folder Name
FigS5D_AP5_141104
File Format
.oib
File Size
250MB
File Separation Method
-
Figure Number
FigS5D
Dimensions
256x256x1x1x2000
X Scale
1.99 micrometer
Y Scale
1.99 micrometer
Z Scale
-
T Scale
0.429 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Olfactory bulb slices of M/T-GCaMP3 mouse. The acute slice excised from P2-3 mouse brain. The slice was treated with AP5.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
Intrinsic Variable
A mouse line Thy1-GCaMP6f (C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J) in which GCaMP6f is expressed in neurons.
Extrinsic Variable
The brain slice was treated with 50 micromolar AP5.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J

Treatments

Reagent / Compound
2-amino-5-phosphonopentanoic acid
Concentration
50 micromolar
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-