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289-Fujimoto-MitralCell/FigS3C_P5_20151106 thumbnail

289-Fujimoto-MitralCell/FigS3C_P5_20151106

Author reviewed
Time-lapse calcium imaging of olfactory bulb of P5 mouse in vivo expressing GCaMP6f in mitral/tufted cells
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Project
289-Fujimoto-MitralCell
Dataset ID
FigS3C_P5_20151106
Kind
Image data
Title
Time-lapse calcium imaging of olfactory bulb of P5 mouse in vivo expressing GCaMP6f in mitral/tufted cells
Description
Time-lapse calcium imaging of olfactory bulb of postnatal day 5 (P5) mouse expressing GCaMP6f in mitral/tufted cells. Glomerular layer was imaged and spontaneous activity was observed in awake animal.
Folder Name
FigS3C_P5_20151106
File Format
.oib
File Size
134MB, 750MB
File Separation Method
data of 2 sessions from one pup
Figure Number
FigS3C
Dimensions
256x256x1x1x1073, 256x256x1x1x6000
X Scale
1.99 micrometer
Y Scale
1.99 micrometer
Z Scale
-
T Scale
0.429 seconds 0.429 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Live and awake P5 mouse expressing GCaMP6f in mitral/tufted cells
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
Intrinsic Variable
A mouse line Thy1-GCaMP6f (C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J) in which GCaMP6f is expressed in neurons.
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XXLPLN25XWMP, NA = 1.05, WD = 2.0
Filter Set
-
Dichroic
-