SSBD:database

289-Fujimoto-MitralCell/FigS3B_Isoflurane_during thumbnail

289-Fujimoto-MitralCell/FigS3B_Isoflurane_during

Author reviewed
Time-lapse calcium imaging of olfactory bulb of anesthetized mouse in vivo expressing GCaMP6f in mitral/tufted cells
SSBD:OMERO.gallery (Project) SSBD:OMERO (Project) SSBD:OMERO.gallery (Dataset) SSBD:OMERO (Dataset)
SSBD:downloads
Project
289-Fujimoto-MitralCell
Dataset ID
FigS3B_Isoflurane_during
Kind
Image data
Title
Time-lapse calcium imaging of olfactory bulb of anesthetized mouse in vivo expressing GCaMP6f in mitral/tufted cells
Description
Time-lapse calcium imaging of olfactory bulb of anesthetized mouse at P7 expressing GCaMP6f in mitral/tufted cells. The pup was anesthetized with Isoflurane. Glomerular layer was imaged and spontaneous activity was observed. The images include the neural activity during anesthesia.
Folder Name
FigS3B_Isoflurane_during
File Format
.oib
File Size
625MB
File Separation Method
-
Figure Number
FigS3B
Dimensions
256x256x1x1x5000
X Scale
1.99 micrometer
Y Scale
1.99 micrometer
Z Scale
-
T Scale
0.429 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Live and anesthetized P4 mouse expressing GCaMP6f in mitral/tufted cells
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
Intrinsic Variable
A mouse line Thy1-GCaMP6f (C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J) in which GCaMP6f is expressed in neurons.
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J

Treatments

Reagent / Compound
isoflurane
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XXLPLN25XWMP, NA = 1.05, WD = 2.0
Filter Set
-
Dichroic
-