289-Fujimoto-MitralCell/FigS2K_P6_OSN-TeNT
Author reviewedCalcium imaging of maternally-reared OSN-TeNT mouse crossed with M/T-GCaMP6f mouse
Project
Dataset ID
FigS2K_P6_OSN-TeNT
Kind
Image data
Title
Calcium imaging of maternally-reared OSN-TeNT mouse crossed with M/T-GCaMP6f mouse
Description
Calcium imaging of maternally-reared OSN-TeNT mouse crossed with M/T-GCaMP6f mouse.
Folder Name
FigS2K_P6_OSN-TeNT
File Format
.oib
File Size
175MB
File Separation Method
-
Figure Number
FigS2K
Dimensions
256x256x1x1x1398
X Scale
1.99 micrometer
Y Scale
1.99 micrometer
Z Scale
-
T Scale
0.429 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)
Biosample
Description
Live and awake P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells with expression of OSN-TeNT. In the OSN-TeNT mouse, TeNT (tetanus toxin light chain) was expressed in OSNs (olfactory sensory neurons).
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
A mouse line Thy1-GCaMP6f (C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J) in which GCaMP6f is expressed in neurons. A mouse line expressing tetanus toxin light chain (TeNT) in OSNs (OMP-Cre;R26-CAG-LoxP-TeNT, designated OSN-TeNT)
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J
B6;129P2-Omp^{tm4(cre)Mom}/MomJ
B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-EGFP/TeNT)Imayo>
Gene
-
Protein / Probe
Tetanus toxin
Method / Genotype
transgenic / C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J
B6;129P2-Omp^{tm4(cre)Mom}/MomJ
B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-EGFP/TeNT)Imayo>
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XXLPLN25XWMP, NA = 1.05, WD = 2.0
Filter Set
-
Dichroic
-