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289-Fujimoto-MitralCell/FigS2E_Odor_ctrl_9 thumbnail

289-Fujimoto-MitralCell/FigS2E_Odor_ctrl_9

Author reviewed
Time-lapse images of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells without expression of OSN-TeNT in the olfactory sensory neurons.
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Project
289-Fujimoto-MitralCell
Dataset ID
FigS2E_Odor_ctrl_9
Kind
Image data
Title
Time-lapse images of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells without expression of OSN-TeNT in the olfactory sensory neurons.
Description
Time-lapse images of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells without expression of OSN-TeNT in the olfactory sensory neurons. During imaging, the pups were exposed to an odor stimulus for several tens of seconds. For odor stimululation, Amyl-acetate diluted in mineral oil was delivered by vapor (1/1000).
Folder Name
FigS2E_Odor_ctrl_9
File Format
.oib
File Size
73MB, 128KB
File Separation Method
2 series
Figure Number
FigS2E
Dimensions
256x8x1x1x18657, 256x256x1x1x1
X Scale
1.99 micrometer
Y Scale
1.99 micrometer
Z Scale
-
T Scale
0.032 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Live and awake P6 mouse expressing GCaMP6f in mitral/tufted cells
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
Intrinsic Variable
A mouse line Thy1-GCaMP6f (C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J) in which GCaMP6f is expressed in neurons.
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
response to odorant (GO:1990834)
Cellular Component
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J

Treatments

Reagent / Compound
pentyl acetate
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Brain (D001921)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XXLPLN25XWMP, NA = 1.05, WD = 2.0
Filter Set
-
Dichroic
-