289-Fujimoto-MitralCell/FigS2E_Odor_OSN-TeNT_4
Author reviewedTime-lapse images of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells with expression of OSN-TeNT in the olfactory sensory neurons.
Project
Dataset ID
FigS2E_Odor_OSN-TeNT_4
Kind
Image data
Title
Time-lapse images of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells with expression of OSN-TeNT in the olfactory sensory neurons.
Description
Time-lapse images of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells with expression of OSN-TeNT. In the OSN-TeNT mouse, TeNT (tetanus toxin light chain) was expressed in OSNs (olfactory sensory neurons). During imaging, the pups were exposed to an odor stimulus for several tens of seconds. For odor stimululation, Amyl-acetate diluted in mineral oil was delivered by vapor (1/1000).
Folder Name
FigS2E_Odor_OSN-TeNT_4
File Format
.oib
File Size
175MB
File Separation Method
-
Figure Number
FigS2E
Dimensions
256x256x1x1x1398
X Scale
1.99 micrometer
Y Scale
1.99 micrometer
Z Scale
-
T Scale
0.429 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)
Biosample
Description
Live and awake P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells with expression of OSN-TeNT. In the OSN-TeNT mouse, TeNT (tetanus toxin light chain) was expressed in OSNs (olfactory sensory neurons).
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
A mouse line Thy1-GCaMP6f (C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J) in which GCaMP6f is expressed in neurons. A mouse line expressing tetanus toxin light chain (TeNT) in OSNs (OMP-Cre;R26-CAG-LoxP-TeNT, designated OSN-TeNT)
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
response to odorant (GO:1990834)
Cellular Component
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J
B6;129P2-Omp^{tm4(cre)Mom}/MomJ
B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-EGFP/TeNT)Imayo>
Gene
-
Protein / Probe
Tetanus toxin
Method / Genotype
transgenic / C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J
B6;129P2-Omp^{tm4(cre)Mom}/MomJ
B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-EGFP/TeNT)Imayo>
Treatments
Reagent / Compound
pentyl acetate
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XXLPLN25XWMP, NA = 1.05, WD = 2.0
Filter Set
-
Dichroic
-