289-Fujimoto-MitralCell/FigS2B_P6_CNGA2mut_1
Author reviewedZ-series images of CNGA2 mutant mouse brain in which mitral cells were labed by tdTomato
Project
Dataset ID
FigS2B_P6_CNGA2mut_1
Kind
Image data
Title
Z-series images of CNGA2 mutant mouse brain in which mitral cells were labed by tdTomato
Description
Z-series images of CNGA2 (a cyclic nucleotide gated channel subunit) mutant mouse brain at P6 in which mitral cells were labed by tdTomato. CNGA2 was specifically knocked out in olfactory sensory neurons of the mouse to examine the role of neural activity generated by olfactory signal transduction.
Folder Name
FigS2B_P6_CNGA2mut_1
File Format
.oif
File Size
666MB
File Separation Method
-
Figure Number
FigS2B
Dimensions
3070x1024x111x1x1
X Scale
0.497 micrometer
Y Scale
0.497 micrometer
Z Scale
10 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Paper(s)
Biosample
Description
A fixed and cleared olfactory bulb of P6 mouse. tdTomato was expressed in mitral cells. CNGA2 (a cyclic nucleotide gated channel subunit) was specifically knocked out in olfactory sensory neurons
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
floxed-Cnga2 and OMP-Cre mice, in which CNGA2 was knocked out in olfactory sensory neurons.
Extrinsic Variable
By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCAFNF-tdTomato), and pCAG-FLPo.
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development neuron remodeling (GO:0016322)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transfection
electroporation / B6;129P2-Omp^{tm4(cre)Mom}/MomJ
floxed-Cnga2
Gene
Cnga2
Protein / Probe
-
Method / Genotype
transgenic / B6;129P2-Omp^{tm4(cre)Mom}/MomJ
floxed-Cnga2
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
tdTomatoFP
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
vibratome-sectioned tissue
tissue clearing
MeSH
Instrument
Body
Olympus, FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XSVMP, NA 1.0, WD 4.0 mm
Filter Set
-
Dichroic
-