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289-Fujimoto-MitralCell/FigS1K_E10_Kir_2 thumbnail

289-Fujimoto-MitralCell/FigS1K_E10_Kir_2

Author reviewed
Z-series images of mouse olfactory bulb with overexpression of Kir2.1 in mitral cells
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Project
289-Fujimoto-MitralCell
Dataset ID
FigS1K_E10_Kir_2
Kind
Image data
Title
Z-series images of mouse olfactory bulb with overexpression of Kir2.1 in mitral cells
Description
Z-series immunostaining images of P6 mouse brain. By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCALNL-tdTomato) and a small amount of Cre plasmid (pCAG-Cre) at E10 to label a subset of mitral cells sparsely. pCALNL-Kir2.1 was also elactroporated to overexpress Kir2.1, and reduce the activity of mitral cells. The brain was sliced and cleared with SeeDB2, and staind with DAPI.
Folder Name
FigS1K_E10_Kir_2
File Format
.lif
File Size
1.92GB
File Separation Method
include 3 series of data
Figure Number
FigS1K
Dimensions
1024x1024x239x2x1 1024x1024x239x2x1 1025x1870x239x2x1
X Scale
0.57 micrometer
Y Scale
0.57 micrometer
Z Scale
2 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
A fixed and cleared olfactory bulb of mouse at P6. tdTomato and Kir2.1 were expressed in mitral/tufted cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
ICR (https://www.jslc.co.jp/pdf/mouse/002_ICR2013.pdf)
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
Intrinsic Variable
-
Extrinsic Variable
By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCALNL-tdTomato), Cre plasmid (pCAG-Cre), and a plasmid for overexpression of Kir2.1 (pCALNL-Kir2.1) at E10.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development neuron remodeling (GO:0016322)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation
Gene
-
Protein / Probe
-4',6-diamidino-2-phenylindole (DAPI)
Method / Genotype
-
Gene
-
Protein / Probe
Inward rectifier potassium channel 2
Method / Genotype
transfection electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
tdTomatoFP DAPI
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue vibratome-sectioned tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Instrument

Body
Leica DMi8
Module
-
Light Source
-
Detector
-
Objective
Leica HC PL APO 20x/0,75 IMM CORR CS2
Filter Set
-
Dichroic
-