SSBD:database

289-Fujimoto-MitralCell/FigS1E_flagKir thumbnail

289-Fujimoto-MitralCell/FigS1E_flagKir

Author reviewed
Z-series images showing the expression of FLAG-Kir2.1 in tdTomato-labeled mitral cells
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Project
289-Fujimoto-MitralCell
Dataset ID
FigS1E_flagKir
Kind
Image data
Title
Z-series images showing the expression of FLAG-Kir2.1 in tdTomato-labeled mitral cells
Description
Z-series immunostaining images of P6 mouse brain. By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCALNL-tdTomato) and a small amount of Cre plasmid (pCAG-Cre) at E12 to label a subset of mitral cells sparsely. pCALNL-Kir2.1 was also elactroporated to overexpress Kir2.1, and reduce the activity of mitral cells. The brain was sliced and cleared with SeeDB2, and staind for Kir2.1 and DAPI.
Folder Name
FigS1E_Kir
File Format
.lif
File Size
60MB
File Separation Method
-
Figure Number
FigS1E
Dimensions
1024x1024x20x3x1
X Scale
0.568 micrometer
Y Scale
0.568 micrometer
Z Scale
2 micrometer
T Scale
-
Channel
3 channel
Quantitative Data
-
Paper(s)

Biosample

Description
A fixed and cleared olfactory bulb of P6 mouse. tdTomato and FLAG-Kir2.1 were expressed in mitral cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
ICR (https://www.jslc.co.jp/pdf/mouse/002_ICR2013.pdf)
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
Intrinsic Variable
-
Extrinsic Variable
By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCALNL-tdTomato) and Cre plasmid (pCAG-Cre) at E12.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
-
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation
Gene
-
Protein / Probe
Inward rectifier potassium channel 2 / antibody anti-FLAG antibody
Method / Genotype
transfection electroporation
Gene
-
Protein / Probe
-4',6-diamidino-2-phenylindole (DAPI)
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
tdTomatoFP DAPI primary antibody plus labeled secondary antibody
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue, cyrostat-sectioned tissue,
MeSH
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Instrument

Body
Leica DMi8
Module
-
Light Source
-
Detector
-
Objective
Leica HC PL APO 20x/0,75 IMM CORR CS2
Filter Set
-
Dichroic
-