SSBD:database

289-Fujimoto-MitralCell/FigS1D_ICR_P5_3 thumbnail

289-Fujimoto-MitralCell/FigS1D_ICR_P5_3

Author reviewed
Z-series images of P5 mouse olfactory bulb, in which mitral cell were labeled
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Project
289-Fujimoto-MitralCell
Dataset ID
FigS1D_ICR_P5_3
Kind
Image data
Title
Z-series images of P5 mouse olfactory bulb, in which mitral cell were labeled
Description
Z-series images of P5 mouse olfactory bulb, in which mitral cell were labeled. By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCALNL-tdTomato) and a small amount of Cre plasmid (pCAG-Cre) at E12 to label a subset of mitral cells sparsely. At P5 the brain was sliced and cleared with SeeDB2, After DAPI staining the imaging was conducted. The mouse strain, ICR was used.
Folder Name
FigS1D_ICR_P5_3
File Format
.lif
File Size
1.7GB
File Separation Method
include 2 series of data
Figure Number
FigS1D
Dimensions
1024x1024x218x2x1
X Scale
0.568 micrometer
Y Scale
0.568 micrometer
Z Scale
2 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
A fixed and cleared olfactory bulb of ICR mouse at P5. tdTomato was expressed in mitral/tufted cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
ICR (https://www.jslc.co.jp/pdf/mouse/002_ICR2013.pdf)
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
Intrinsic Variable
-
Extrinsic Variable
By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCALNL-tdTomato) and Cre plasmid (pCAG-Cre) at E12.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development neuron remodeling (GO:0016322)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation
Gene
-
Protein / Probe
-4',6-diamidino-2-phenylindole (DAPI)
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
tdTomatoFP 4',6-diamidino-2-phenylindole (DAPI)
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue vibratome-sectioned tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Instrument

Body
Leica DMi8
Module
-
Light Source
-
Detector
-
Objective
Leica HC PL APO 20x/0,75 IMM CORR CS2
Filter Set
-
Dichroic
-