SSBD:database

289-Fujimoto-MitralCell/Fig7_BarrelCortex_RhoABC-KO_1

Author reviewed

Z-series images of RhoABC knockout mouse brain at P14 in which layer 4 neurons were labed

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Project

289-Fujimoto-MitralCell

Dataset ID

Fig7_BarrelCortex_RhoABC-KO_1

Kind

Image data

Title

Z-series images of RhoABC knockout mouse brain at P14 in which layer 4 neurons were labed

Description

Z-series images of the barrel cortex of RhoA, RhoB, and RhoC knockout mouse at P14 in which layer 4 neurons were labed by tdTomato. RhoA, B, and C were knocked out by using CRISPR-Cas9 and in utero electroporation. VGluT2 was visualized by immmunohistochemistry.

Folder Name

Fig7_BarrelCortex_RhoABC-KO_1

File Format

.lif

File Size

42GB

File Separation Method

10 series of images

Figure Number

Fig7

Dimensions

2048x2048x100x3x1

X Scale

0.284 micrometer

Y Scale

0.284 micrometer

Z Scale

1.04 micrometer

T Scale

Channel

3 channel

Quantitative Data

Paper(s)

Biosample

Description

A fixed and cleared barrel cortex of P14 mouse in which RhoA, RhoB, and RhoC were knocked out. tdTomato was expressed in layer 4 neurons.

Organism

Mus musculus (NCBITaxon:10090)

Strain

ICR (https://www.jslc.co.jp/pdf/mouse/002_ICR2013.pdf)

Cell

neuron (CL:0000540)

Cell line

MeSH

Neurons (D009474)

Microscopy, Confocal (D018613)

Cerebral Cortex (D002540)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

Intrinsic Variable

pCAX-hCas9 and the gRNA for RhoA, RhoB, and RhoC were introduced to knockout RhoA, RhoB, and RhoC by CRISPR-Cas9 method.

Extrinsic Variable

By using in utero electroporation, the authors electroporated pCAFNF-tdTomato and pCAG-FLPo at E13.5.

Ontology

Anatomical Entity

UBERON

barrel cortex (UBERON:0010415)

cerebral cortex (UBERON:0000956)

brain (UBERON:0000955)

Biological Process

neuron development neuron remodeling (GO:0016322)

Cellular Component

cell body (GO:0044297)

dendrite (GO:0030425)

Molecular Function

MeSH Anatomy

Neurons (D009474)

Microscopy, Confocal (D018613)

Cerebral Cortex (D002540)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

MeSH BP

Neurons (D009474)

Microscopy, Confocal (D018613)

Cerebral Cortex (D002540)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

MeSH CC

Neurons (D009474)

Microscopy, Confocal (D018613)

Cerebral Cortex (D002540)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

MeSH MF

Neurons (D009474)

Microscopy, Confocal (D018613)

Cerebral Cortex (D002540)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

Genetics

Gene

Protein / Probe

Method / Genotype

transfection electroporation

Gene

Protein / Probe

Vesicular glutamate transporter 2 / antibody

Method / Genotype

Gene

Rhoa

Protein / Probe

Method / Genotype

gene knock out electroporation

Gene

Rhob

Protein / Probe

Method / Genotype

gene knock out electroporation

Gene

Rhoc

Protein / Probe

Method / Genotype

gene knock out electroporation

Treatments

Reagent / Compound

Concentration

Fold Dilution

Imaging Method

confocal microscopy (FBbi:00000251)

Detection Method

Visualization Method

tdTomatoFP primary antibody plus labeled secondary antibody

Illumination Method

Source of Contrast

distribution of a specific protein

Imaged Parameter

fluorescence emission

Sample Preparation Method

formaldehyde fixed tissue vibratome-sectioned tissue tissue clearing

MeSH

Neurons (D009474)

Microscopy, Confocal (D018613)

Cerebral Cortex (D002540)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

Instrument

Body

Leica DMi8

Module

Light Source

Detector

Objective

Leica HC PL APO 20x/0,75 IMM CORR CS2

Filter Set

Dichroic